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Anti-SHANK3 antibody [N69/46] (ab93607)

Key features and details

  • Mouse monoclonal [N69/46] to SHANK3
  • Suitable for: WB, IHC-P, Flow Cyt
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG2b

Overview

  • Product name

    Anti-SHANK3 antibody [N69/46]
    See all SHANK3 primary antibodies

  • Description

    Mouse monoclonal [N69/46] to SHANK3

  • Host species

    Mouse

  • Specificity

    No cross-reactivity with SHANK1 or SHANK2.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IHC-P
    Mouse
    WB
    Rat
    See all applications and species data

  • Immunogen

    Synthetic peptide:

    PEKLPGSLRKGIPRTKSV

    , corresponding to internal sequence amino acids 840 - 857 of Rat SHANK3 (Acc. No. Q9JLU4).
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • General notes

    The clone number has been updated from S69-46 to N69/46, both clone numbers name the same antibody clone.

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-SHANK3 antibody [N69/46] (ab93607)
    Western blot - Anti-SHANK3 antibody [N69/46] (ab93607)
    Anti-SHANK3 antibody [N69/46] (ab93607) at 1/1000 dilution + Rat brain membrane lysate at 15 µg

    Secondary
    Sheep Anti-Mouse IgG-HRP

    Predicted band size: 186 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK3 antibody [N69/46] (ab93607)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SHANK3 antibody [N69/46] (ab93607)

    Immunohistochemical analysis of mouse backskin tissue sections labling SHANK3 with ab93607 at 1/100 dilution. FITC Goat anti-mouse at 1/50 was used as the secondary antibody. 

     

  • Flow Cytometry - Anti-SHANK3 antibody [N69/46] (ab93607)
    Flow Cytometry - Anti-SHANK3 antibody [N69/46] (ab93607)
    Overlay histogram showing SH-SY5Y cells stained with ab93607 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab93607, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in SH-SY5Y cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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