Anti-SPACA1 antibody - C-terminal (ab191843)
Key features and details
- Rabbit polyclonal to SPACA1 - C-terminal
- Suitable for: ICC/IF, WB
- Reacts with: Mouse
- Isotype: IgG
Overview
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Product name
Anti-SPACA1 antibody - C-terminal -
Description
Rabbit polyclonal to SPACA1 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse
Predicted to work with: Cow
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Immunogen
Synthetic peptide corresponding to Mouse SPACA1 aa 250 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
Database link: Q9DA48 -
Positive control
- Extracts from testis of wild type (+/+) and SPACA1 single (+/-) knock-out mice; mouse spermatids.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-SPACA1 antibody - C-terminal (ab191843)All lanes : Anti-SPACA1 antibody - C-terminal (ab191843) at 1/1000 dilution
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse sperm cell lysate
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 33 kDaSeveral proteins associated with sperm undergo modification during maturation. Multiple bands are reflection of post-translational modifications by glycosylation and phosphorylation.
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Western blot - Anti-SPACA1 antibody - C-terminal (ab191843)All lanes : Anti-SPACA1 antibody - C-terminal (ab191843) at 1/1000 dilution
Lane 1 : extracts from testis of wild type (+/+) mice
Lane 2 : extracts from testis of SPACA1 single (+/-) knock-out mice
Lane 3 : extracts from testis of SPACA1 double (-/-) knock-out mice
Predicted band size: 33 kDaBasigin was used as a loading control.
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Immunocytochemistry/ Immunofluorescence - Anti-SPACA1 antibody - C-terminal (ab191843)Immunofluorescent analysis of round spermatids labeling SPACA1 with ab191843 at 1/100 and as a secondary antibody, Alexa-Fluor 488 conjugated anti-rabbit IgG antibody. Image at the right stained with Hoechst 33342.
