Anti-Ki67 antibody [BLR021E] (ab243878)
![Anti-Ki67 antibody [BLR021E] (ab243878)](https://www.abcam.com/ps/products/243/ab243878/Images/ab243878-334303-anti-ki67-antibody-blr021e-western-blot.jpg "Anti-Ki67 antibody [BLR021E] (ab243878)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [BLR021E] to Ki67
- Suitable for: WB, IHC-P, ICC
- Reacts with: Human
Overview
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Product name
Anti-Ki67 antibody [BLR021E]
See all Ki67 primary antibodies -
Description
Rabbit monoclonal [BLR021E] to Ki67 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC HumanIHC-P HumanWB Human
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Immunogen
Synthetic peptide within Human Ki67 aa 1200-1250. The exact sequence is proprietary. Corresponds to the repetitive 66 bp element. NP_002408.2 and Gene ID 4288.
Database link: P46013 -
General notes
This product is sold under License from Bethyl Laboratories, Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.8
Preservative: 0.09% Sodium azide
Constituents: 98% Borate buffered saline, 0.1% BSA -
Concentration information loading... -
Purification notes
Recombinant antibody was purified from cell culture supernatant. -
Clonality
Monoclonal -
Clone number
BLR021E -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Ki67 antibody [BLR021E] (ab243878)
Western blot analysis of Ki67 using ab243878 at 1/1000 dilution.
Lane 1: HeLa whole cell lysate (50 µg).
Lane 2: HEK-293T whole cell lysate (50 µg).
A HRP-conjugated goat anti-rabbit IgG antibody was used as the secondary. Detection: chemiluminescence with an exposure time of 10 seconds.
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![Immunocytochemistry - Anti-Ki67 antibody [BLR021E] (ab243878)](https://www.abcam.com/ps/products/243/ab243878/Images/ab243878-334302-anti-ki67-antibody-blr021e-immunocytochemistry.jpg)
Immunocytochemistry - Anti-Ki67 antibody [BLR021E] (ab243878)Paraffin-embedded, Formaldehyde-fixed OVCAR-8 cells labeling Ki67 using ab243878 at 1/250 dilution in ICC/IF analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary antibody. DAB staining -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)](https://www.abcam.com/ps/products/243/ab243878/Images/ab243878-334301-anti-ki67-antibody-blr021e-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Ki67 antibody [BLR021E] (ab243878)Formalin-fixed, paraffin-embedded human tonsil tissue stained for Ki67 using ab243878 at 1/250 dilution in immunohistochemical analysis. A HRP-conjugated goat anti-rabbit IgG was used as the secondary. DAB staining. -
![Anti-Ki67 antibody [BLR021E] (ab243878)](https://www.abcam.com/ps/products/243/ab243878/Images/ab243878-4-benefits-of-recombinant-antibodies.png)
Anti-Ki67 antibody [BLR021E] (ab243878)
