Anti-PRAS40 antibody [EPR6262] (ab134158)
![Anti-PRAS40 antibody [EPR6262] (ab134158)](https://www.abcam.com/ps/products/134/ab134158/Images/ab134158-319570-ab134158KOWBDWAR.jpg "Anti-PRAS40 antibody [EPR6262] (ab134158)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6262] to PRAS40
- Suitable for: WB
- Knockout validated
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-PRAS40 antibody [EPR6262]
See all PRAS40 primary antibodies -
Description
Rabbit monoclonal [EPR6262] to PRAS40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseHuman
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Immunogen
Synthetic peptide corresponding to residues near the C terminus of Human PRAS40 (UniProt Q96B36).
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Positive control
- NIH/3T3, L6, HeLa, and 293T cell lysates
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR6262 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRAS40 antibody [EPR6262] (ab134158)All lanes : Anti-PRAS40 antibody [EPR6262] (ab134158) at 1/1000 dilution
Lane 1 : Wild-type HAP1 whole cell lysate
Lane 2 : AKT1S1 knockout HAP1 whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : MCF-7 whole cell lysate
Lysates/proteins at 20 µg per lane.
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?Lanes 1 - 4: Merged signal (red and green). Green - ab134158 observed at 27 kDa. Red - loading control, ab9484, observed at 37 kDa.
ab134158 was shown to specifically react with PRAS40 in wild-type HAP1 cells as signal was lost in AKT1S1 knockout cells. Wild-type and AKT1S1 knockout samples were subjected to SDS-PAGE. Ab134158 and ab9484 (Mouse anti-GAPDH loading control) were incubated overnight at 4°C at 1/1000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
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![Western blot - Anti-PRAS40 antibody [EPR6262] (ab134158)](https://www.abcam.com/ps/products/134/ab134158/Images/PRAS40-Primary-antibodies-ab134158-2.jpg)
Western blot - Anti-PRAS40 antibody [EPR6262] (ab134158)All lanes : Anti-PRAS40 antibody [EPR6262] (ab134158) at 1/1000 dilution
Lane 1 : NIH/3T3 cell lysate
Lane 2 : L6 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
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![Anti-PRAS40 antibody [EPR6262] (ab134158)](https://www.abcam.com/ps/products/134/ab134158/Images/ab134158-3-benefits-of-recombinant-antibodies.png)
Anti-PRAS40 antibody [EPR6262] (ab134158)
