Porcine IL-17F ELISA Kit (ab273224)
Key features and details
- Sensitivity: 6 pg/ml
- Range: 4.1 pg/ml - 1000 pg/ml
- Sample type: Cell culture supernatant, Cit plasma, EDTA Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Rat, Pig
Overview
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Product name
Porcine IL-17F ELISA Kit
See all IL-17F kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall Inter-assay Sample n Mean SD CV% Overall -
Sample type
Cell culture supernatant, Serum, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
6 pg/ml -
Range
4.1 pg/ml - 1000 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Cell culture supernatant 76.04 73% - 84% Serum 74.84 63% - 71% Plasma 82.75 75% - 91% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Rat, Pig -
Product overview
Porcine IL-17F ELISA Kit (ab273224) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Porcine IL-17F in serum, plasma (Collect plasma using EDTA and citrate as an anti-coagulant. Heparin is not recommended as anti-coagulant for use in this assay) and cell culture supernatants.
This assay employs an antibody specific for Porcine IL-17F coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17F present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Porcine IL-17F antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17F bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 150X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml 5X Assay Diluent D 1 x 15ml Anti-Porcine IL-17F coated Microplate (12 x 8 wells) 1 unit Biotinylated Anti-Porcine IL-17F Antibody 2 vials Porcine IL-17F Standard (Lyophilized) 2 vials Stop Solution 1 x 8ml TMB Substrate Solution 1 x 12ml -
Research areas
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Function
Ligand for IL17RA and IL17RC (PubMed:17911633). The heterodimer formed by IL17A and IL17F is a ligand for the heterodimeric complex formed by IL17RA and IL17RC (PubMed:18684971). Involved in stimulating the production of other cytokines such as IL6, IL8 and CSF2, and in regulation of cartilage matrix turnover (PubMed:11591732, PubMed:11591768, PubMed:11574464). Also involved in stimulating the proliferation of peripheral blood mononuclear cells and T-cells and in inhibition of angiogenesis (PubMed:11591732). Plays a role in the induction of neutrophilia in the lungs and in the exacerbation of antigen-induced pulmonary allergic inflammation. -
Tissue specificity
Expressed in activated, but not resting, CD4+ T-cells and activated monocytes. -
Involvement in disease
Candidiasis, familial, 6 -
Sequence similarities
Belongs to the IL-17 family. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- CANDF6
- Cytokine ML 1
- Cytokine ML-1
see all -
Database links
- Entrez Gene: 100037933 Pig
- Entrez Gene: 301291 Rat
- SwissProt: Q5BJ95 Rat
- Unigene: 34022 Rat
Images
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Porcine IL-17F Standard Curve
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
Porcine IL-17F ELISA kit (ab273224) Standard curve.