Anti-PRAS40 antibody (ab72321)
Key features and details
- Rabbit polyclonal to PRAS40
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-PRAS40 antibody
See all PRAS40 primary antibodies -
Description
Rabbit polyclonal to PRAS40 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species IP HumanWB Human
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Immunogen
Synthetic peptide corresponding to a region between residues 195 and 245 of human PRAS40, (NP_115751.1).
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Positive control
- HeLa and 293T whole cell lysates.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-PRAS40 antibody (ab72321)All lanes : Anti-PRAS40 antibody (ab72321) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Predicted band size: 40 kDa
Observed band size: 40 kDa
Additional bands at: 30 kDa, 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 30 seconds
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Immunoprecipitation - Anti-PRAS40 antibody (ab72321)Detection of PRAS40 by Western Blot of Immunprecipitate.
ab72321 at 1µg/ml for WB, staining PRAS40 in HeLa whole cell lysate subsequent to immunoprecipitation using ab72321 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
