Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)
![Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)](https://www.abcam.com/ps/products/248/ab248559/Images/ab248559-405961-anti-casein-kinase-2-beta-antibody-epr1994-bsa-and-azide-free-western-blot.jpg "Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta - BSA and Azide free
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free
See all Casein Kinase 2 beta primary antibodies -
Description
Rabbit monoclonal [EPR1994] to Casein Kinase 2 beta - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details
Unsuitable for: Flow Cyt or ICC -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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General notes
Ab248559 is the carrier-free version of ab133576. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
ab248559 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Dissociation constant (KD)
KD = 1.94 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR1994 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)All lanes : Anti-Casein Kinase 2 beta antibody [EPR1994] (ab133576) at 1/1000 dilution
Lane 1 : HepG2 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lane 4 : HUVEC cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 24 kDa
Observed band size: 25 kDa why is the actual band size different from the predicted?This data was developed using ab133576, the same antibody clone in a different buffer formulation.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)](https://www.abcam.com/ps/products/248/ab248559/Images/ab248559-2-anti-casein-kinase-2-beta-antibody-epr1994-bsa-and-azide-free-immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)This data was developed using ab133576, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of Casein Kinase 2 beta in formalin fixed, paraffin embedded Human breast tissue, using ab133576 at a 1/50 dilution. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. -
![OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)](https://www.abcam.com/ps/products/248/ab248559/Images/ab248559-1-anti-casein-kinase-2-beta-antibody-epr1994-bsa-and-azide-free-equilibrium-disassociation-constant-kd.jpg)
OI-RD Scanning - Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)This data was developed using ab133576, the same antibody clone in a different buffer formulation.Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
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![Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)](https://www.abcam.com/ps/products/248/ab248559/Images/ab248559-4-benefits-of-recombinant-antibodies.png)
Anti-Casein Kinase 2 beta antibody [EPR1994] - BSA and Azide free (ab248559)
