Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR14489(2)] to NSDHL - BSA and Azide free
  • Suitable for: WB, IP
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free
    See all NSDHL primary antibodies

  • Description

    Rabbit monoclonal [EPR14489(2)] to NSDHL - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293T, A431, HeLa, NIH/3T3, PC-12, C6 and HepG2 cell lysates; Human fetal brain tissue lysate. IP: Fetal brain tissue lysate; HeLa cell lysate.

  • General notes

    Ab251292 is the carrier-free version of ab199730. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251292 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Western blot - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    Western blot - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

    Lane 1 : Wild-type HEK293T cell lysate
    Lane 2 : NSDHL knockout HEK293T cell lysate
    Lane 3 : A431 cell lysate
    Lane 4 : HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution

    Predicted band size: 42 kDa
    Observed band size: 38 kDa
    why is the actual band size different from the predicted?



    This data was developed using ab199730, the same antibody clone in a different buffer formulation.

    Lanes 1-4: Merged signal (red and green). Green - ab199730 observed at 38 kDa. Red - loading control ab7291 observed at 50 kDa.

     ab199730 Anti-NSDHL antibody [EPR14489(2)] was shown to specifically react with NSDHL in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266682 (knockout cell lysate ab258082) was used. Wild-type and NSDHL knockout samples were subjected to SDS-PAGE. ab199730 and Anti-alpha Tubulin antibody [DM1A] - Loading Control (ab7291) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Western blot - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    Western blot - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

    Lane 1 : A431 (Human epidermoid carcinoma) whole cell lysate
    Lane 2 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate
    Lane 3 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
    Lane 4 : Human fetal brain tissue lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 5 seconds


    This data was developed using ab199730, the same antibody clone in a different buffer formulation.

    Blocking and dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    Western blot - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    All lanes : Anti-NSDHL antibody [EPR14489(2)] (ab199730) at 1/1000 dilution

    Lane 1 : NIH/3T3 (Mouse embryonic fibroblast cell line) whole cell lysate
    Lane 2 : PC-12 (Rat adrenal gland pheochromocytoma cell line) whole cell lysate
    Lane 3 : C6 (Rat glial tumor cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 42 kDa
    Observed band size: 42 kDa


    Exposure time: 10 seconds


    This data was developed using ab199730, the same antibody clone in a different buffer formulation.

    Blocking  buffer: 5% NFDM/TBST.

    Dilution buffer: 5% NFDM /TBST or 1%BSA /TBST.

  • Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    This data was developed using ab199730, the same antibody clone in a different buffer formulation.NSDHL was immunoprecipitated from 1mg of Human fetal brain whole cell lysate with ab199730 at 1/50 dilution. Lane 1: Human fetal brain whole cell lysate 10ug (Input).Lane 2: ab199730 IP in Human fetal brain whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199730 in Human fetal brain whole cell lysate.Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution.Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 5 seconds.
  • Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    Immunoprecipitation - Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    This data was developed using ab199730, the same antibody clone in a different buffer formulation.NSDHL was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysate with ab199730 at 1/50 dilution.Lane 1: HeLa whole cell lysate 10ug (Input).Lane 2: ab199730 IP in HeLa whole cell lysate.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199730 in HeLa whole cell lysate.Western blot was performed from the immunoprecipitate using ab199730 at 1/1000 dilution (Panel A) or ab190353 at 1/1000 dilution (Panel B).Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500.Blocking and dilution buffer and concentration: 5% NFDM/TBST.Exposure time: 30 seconds (Panel A and B). ab199730 was used to immunoprecipitate NSDHL. These precipitates were resolved with ab190353.
  • Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)
    Anti-NSDHL antibody [EPR14489(2)] - BSA and Azide free (ab251292)

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