Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric (ab281907)
![Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric (ab281907)](https://www.abcam.com/ps/products/281/ab281907/Images/ab281907-2-AntiTNFRSF14-HVEM-immunocytochemistry-RAW2647-cells.jpg "Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric (ab281907)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [HMHV-1B18] to TNFRSF14/HVEM - Chimeric
- Suitable for: WB, ICC
- Reacts with: Mouse
Overview
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Product name
Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric
See all TNFRSF14/HVEM primary antibodies -
Description
Rabbit monoclonal [HMHV-1B18] to TNFRSF14/HVEM - Chimeric -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICCmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Fusion protein corresponding to Mouse TNFRSF14/HVEM. Raised by immunizing Armenian hamsters with mouse HVEM:Fc fusion protein
Database link: Q71F55 -
Positive control
- ICC: RAW264.7 cells. WB: Mouse spleen, thymus and lung tissue lysates.
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General notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.02% Proclin 300
Constituent: 99% PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
HMHV-1B18 -
Isotype
IgG -
Light chain type
kappa -
Research areas
Images
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Immunocytochemistry - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281907)
Immunofluorescence analysis of paraformaldehyde fixed RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) cells labelling TNFRSF14/HVEM with ab281907 at 10 µg/ml for 1 hour, followed by Alexa Fluor® 488 secondary antibody at 2 µg/ml (green/top-left). Image showing membrane staining. The nuclear counter stain is DAPI (blue). The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor® 488 secondary antibody. RAW264.7 cells were fixed Shi-fix™ coverslips.
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![Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281907)](https://www.abcam.com/ps/products/281/ab281907/Images/ab281907-1-AntiTNFRSF14-HVEM-westernblot-spleen-thymus-lung-tissue-lysates-mouse.jpg)
Western blot - Anti-TNFRSF14/HVEM antibody [HMHV-1B18] (ab281907)All lanes : Anti-TNFRSF14/HVEM antibody [HMHV-1B18] - Chimeric (ab281907) at 0.01 µg/ml
Lane 1 : Mouse spleen tissue lysate
Lane 2 : Mouse thymus tissue lysate
Lane 3 : Mouse lung tissue lysate
Lysates/proteins at 35 µg per lane.
Secondary
All lanes : Anti-rabbit secondary antibody
Predicted band size: 30 kDaA primary incubation of 1h was used and protein was detected by chemiluminescence.