Acyl-CoA Synthetase Assay Kit (Fluorometric) (ab273315)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate
- Sample type: Adherent cells, Purified protein, Suspension cells, Tissue Extracts, Tissue sections
Overview
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Product name
Acyl-CoA Synthetase Assay Kit (Fluorometric) -
Detection method
Colorimetric/Fluorometric -
Sample type
Adherent cells, Suspension cells, Tissue Extracts, Purified protein, Tissue sections -
Assay type
Quantitative -
Assay duration
Multiple steps standard assay -
Product overview
Acyl-CoA Synthetase (ACS) Assay Kit (Fluorometric) (ab273315) provides a rapid, sensitive and straightforward way to measure ACS activity in various samples. In the assay, acyl-CoA produced by ACS activity is metabolized by the Enzyme Mix, Developer Mix and Converter Mix to generate an intermediate compound, which reacts with a probe, yielding a fluorescent signal that can be measured with excitation at 535 nm and emission at 587 nm. This assay can detect ACS activity as low as 5 mU/µl sample.
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Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Tested applications
Suitable for: Othermore details -
Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 100 tests ACS Assay Buffer 1 x 25ml ACS Converter (Lyophilized) 1 vial ACS Developer (Lyophilized) 1 vial ACS Enzyme Mix (Lyophilized) 1 vial ACS Positive Control (Lyophilized) 1 vial ACS Probe 1 x 0.2ml ACS Substrate 1 x 1.1ml H2O2 Standard 1 x 100µl
Images
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Example data
Typical standard curve – data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.
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Example dataReaction kinetics of ACS Positive Control.
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Example dataACS activity in Rat liver lysate. Rat liver was prepared according to the described protocol. For activity determination, experiments were run in duplicates. 3.3-13.3 µg protein was loaded per well.