Antibodies, Proteins, Kits and Reagents for Life Science

Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Key features and details

Mouse monoclonal [13C2] to NUP98
Suitable for: WB, ICC/IF
Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
Isotype: IgG1

Product name

Anti-NUP98 antibody [13C2]
See all NUP98 primary antibodies
Description

Mouse monoclonal [13C2] to NUP98
Host species

Mouse
Tested applications

Suitable for: WB, ICC/IFmore details
Species reactivity

Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
Immunogen

This product was produced with the following immunogens:
Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
Sequence:
MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ

Database link: D3KYQ3

Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664 (internal sequence).
Sequence: SNPTQGGGLFGAANPGLGG
Database link: D3KYQ3
Run BLAST with Run BLAST with

Run BLAST with Run BLAST with
Positive control
- HeLa cells and cell extract, Tetrahymena themophila cells and cell extract, Saccharomyces cerevisiae cells and cell extract, Schizosaccharomyces pombe cells and cell extract.
General notes

The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 6
Constituents: 50% Glycerol (glycerin, glycerine), 50% PBS
Concentration information loading...
Purity

Protein G purified
Clonality

Monoclonal
Clone number

13C2
Isotype

IgG1
Light chain type

kappa
Research areas

Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Immunofluorescent analysis of HeLa cells labeling NUP98 with ab179894 at 0.5 µg/ml in PBS (top panel). DAPI staining (middle panel). 4 μg/ml of Alexa488-labeled anti-mouse IgG was used as secondary antibody. The color image represents merged images of ab179894 (green) with DAPI (magenta).
Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894) at 0.4 µg/ml + HeLa cell extract

Secondary
HRP-labeled anti-mouse IgG at 0.4 µg/ml

Developed using the ECL technique.

Predicted band size: 112 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Immunofluorescent analysis of Tetrahymena thermophila cells labeling NUP98 with ab179894 at 0.5 µg/ml (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta).4 μg/ml of Alexa488-labelled anti-mouse IgG was used as secondary antibody. Dotted lines represent the outlines of cells. The insert is a magnified image showing the position of the micronucleus.
Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

All lanes : Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Lane 1 : Tetrahymena thermophila wild type cell extract
Lane 2 : Tetrahymena thermophila cell extract ectopically-expressing GFP-MacNup98A (in addition to endogenous MacNup98A)

Predicted band size: 112 kDa

Open arrowheads represent the positions of MacNup98A. The 125-kDa band corresponds to GFP-MacNup98A. Diamonds and asterisks represent uncharacterized proteins.
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Immunofluorescent analysis of S. pombe cells labeling NUP98 with ab179894 (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.
Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

All lanes : Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Lane 1 : S. pombe cell extract
Lane 2 : NUP98-GFP-expressing S. pombe cell extract

Predicted band size: 112 kDa
Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Immunofluorescent analysis of S. cerevisiae cells labeling NUP98 with ab179894 at 1/100 dilution (top panel). DAPI (middle panel). The color image represents a merged image of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.
Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Supernatant of hybridoma culture medium of ab179894 at 1/10 dilution + S. cerevisiae cell extract

Predicted band size: 112 kDa

ab179894 crossreacts with multiple nucleoporins of S. cerevisiae.
Asterisks represent uncharacterized proteins.

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