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Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

Key features and details

  • Mouse monoclonal [13C2] to NUP98
  • Suitable for: WB, ICC/IF
  • Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe
  • Isotype: IgG1

Overview

  • Product name

    Anti-NUP98 antibody [13C2]
    See all NUP98 primary antibodies

  • Description

    Mouse monoclonal [13C2] to NUP98

  • Host species

    Mouse

  • Tested applications

    Suitable for: WB, ICC/IFmore details

  • Species reactivity

    Reacts with: Human, Saccharomyces cerevisiae, Tetrahymena, Schizosaccharomyces pombe

  • Immunogen

    This product was produced with the following immunogens:
    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 1-29 (N terminal).
    Sequence:

    MFGNTGGGGLFGNTQTQQTGGGLFGQPQQ


    Database link: D3KYQ3

    Synthetic peptide corresponding to Tetrahymena sp. NUP98 aa 646-664 (internal sequence).
    Sequence: SNPTQGGGLFGAANPGLGG
    Database link: D3KYQ3
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • Positive control

    • HeLa cells and cell extract, Tetrahymena themophila cells and cell extract, Saccharomyces cerevisiae cells and cell extract, Schizosaccharomyces pombe cells and cell extract.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Immunofluorescent analysis of HeLa cells labeling NUP98 with ab179894 at 0.5 µg/ml in PBS (top panel). DAPI staining (middle panel). 4 μg/ml of Alexa488-labeled anti-mouse IgG was used as secondary antibody. The color image represents merged images of ab179894 (green) with DAPI (magenta).

  • Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894) at 0.4 µg/ml + HeLa cell extract

    Secondary
    HRP-labeled anti-mouse IgG at 0.4 µg/ml

    Developed using the ECL technique.

    Predicted band size: 112 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Immunofluorescent analysis of Tetrahymena thermophila cells labeling NUP98 with ab179894 at 0.5 µg/ml (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta).4 μg/ml of Alexa488-labelled anti-mouse IgG was used as secondary antibody. Dotted lines represent the outlines of cells. The insert is a magnified image showing the position of the micronucleus.

  • Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    All lanes : Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Lane 1 : Tetrahymena thermophila wild type cell extract
    Lane 2 : Tetrahymena thermophila cell extract ectopically-expressing GFP-MacNup98A (in addition to endogenous MacNup98A)

    Predicted band size: 112 kDa



    Open arrowheads represent the positions of MacNup98A. The 125-kDa band corresponds to GFP-MacNup98A. Diamonds and asterisks represent uncharacterized proteins.

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Immunofluorescent analysis of S. pombe cells labeling NUP98 with ab179894 (top panel). DAPI (middle panel). The color image represents merged images of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

  • Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    All lanes : Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Lane 1 : S. pombe cell extract
    Lane 2 : NUP98-GFP-expressing S. pombe cell extract

    Predicted band size: 112 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Immunocytochemistry/ Immunofluorescence - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)

    Immunofluorescent analysis of S. cerevisiae cells labeling NUP98 with ab179894 at 1/100 dilution (top panel). DAPI (middle panel). The color image represents a merged image of ab179894 (green) with DAPI (magenta). Dotted lines represent the outlines of cells.

  • Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Western blot - Anti-NUP98 antibody [13C2] - BSA and Azide free (ab179894)
    Supernatant of hybridoma culture medium of ab179894 at 1/10 dilution + S. cerevisiae cell extract

    Predicted band size: 112 kDa



    ab179894 crossreacts with multiple nucleoporins of S. cerevisiae.

    Asterisks represent uncharacterized proteins.

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