Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cullin 2/CUL-2 antibody [EPR3104(2)]
    See all Cullin 2/CUL-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide within Human Cullin 2/CUL-2 aa 1-100. The exact sequence is proprietary.
    Database link: Q13617

  • Positive control

    • WB: PC3, 293T, HeLa, Raji cell lysates; mouse and rat brain, heart, kidney and spleen lysates. Flow Cyt: 293T cells.

  • General notes

     

     

    Previously labelled as Cullin 2. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    Western blot - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    All lanes : Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917) at 1/1000 dilution

    Lane 1 : PC3 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : HeLa cell lysate
    Lane 4 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 87 kDa

  • Flow Cytometry - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    Flow Cytometry - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

    Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2/CUL-2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

  • Immunoprecipitation - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    Immunoprecipitation - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

    Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2/CUL-2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
    Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

  • Western blot - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    Western blot - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    All lanes : Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917) at 1/2000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse heart lysate
    Lane 3 : Mouse kidney lysate
    Lane 4 : Mouse spleen lysate
    Lane 5 : Rat brain lysate
    Lane 6 : Rat heart lysate
    Lane 7 : Rat kidney lysate
    Lane 8 : Rat spleen lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 87 kDa


    Exposure time: 3 minutes


    Blocking and Diluting buffer: 5% NFDM/TBST

  • Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)
    Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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