Antibodies, Proteins, Kits and Reagents for Life Science

331 689 ₸ Product size

100 µl 331 689 ₸ 10 µl 137 366 ₸

Order now and get it on Tuesday March 02, 2021

Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2
Suitable for: WB, IP, Flow Cyt
Reacts with: Mouse, Rat, Human

Product name

Anti-Cullin 2/CUL-2 antibody [EPR3104(2)]
See all Cullin 2/CUL-2 primary antibodies
Description

Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human Cullin 2/CUL-2 aa 1-100. The exact sequence is proprietary.
Database link: Q13617
Positive control
- WB: PC3, 293T, HeLa, Raji cell lysates; mouse and rat brain, heart, kidney and spleen lysates. Flow Cyt: 293T cells.
General notes
Previously labelled as Cullin 2.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR3104(2)
Isotype

IgG
Research areas
- Cell Biology
- Apoptosis
- Nucleus
- Other
- Cancer
- Cell Death
- Apoptosis
- Nucleus
- Other

Western blot - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

All lanes : Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917) at 1/1000 dilution

Lane 1 : PC3 cell lysate
Lane 2 : 293T cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : Raji cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 87 kDa
Flow Cytometry - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2/CUL-2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunoprecipitation - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2/CUL-2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.
Western blot - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

All lanes : Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917) at 1/2000 dilution

Lane 1 : Mouse brain lysate
Lane 2 : Mouse heart lysate
Lane 3 : Mouse kidney lysate
Lane 4 : Mouse spleen lysate
Lane 5 : Rat brain lysate
Lane 6 : Rat heart lysate
Lane 7 : Rat kidney lysate
Lane 8 : Rat spleen lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 87 kDa

Exposure time: 3 minutes

Blocking and Diluting buffer: 5% NFDM/TBST
Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] (ab166917)

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