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Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2 - BSA and Azide free
  • Suitable for: WB, IP, Flow Cyt
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free
    See all Cullin 2/CUL-2 primary antibodies

  • Description

    Rabbit monoclonal [EPR3104(2)] to Cullin 2/CUL-2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, IP, Flow Cytmore details
    Unsuitable for: ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: PC3, 293T, HeLa, Raji cell lysates; mouse and rat brain, heart, kidney and spleen lysates. Flow Cyt: 293T cells.

  • General notes

    ab240149 is the carrier-free version of ab166917. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab240149 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Flow Cytometry - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)
    Flow Cytometry - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)

    Flow cytometry analysis of 293T (Human embryonic kidney epithelial cell) cells labeling Cullin 2/CUL-2 (red) with ab166917 at a 1/100 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166917).

  • Immunoprecipitation - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)
    Immunoprecipitation - Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)

    Western blot analysis of an immunoprecipitation pellet from Raji cell lysate labeling Cullin 2/CUL-2 with ab166917 which was used at 1/10 dilution for immunoprecipitation.
    Secondary antibody used was an HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab166917).

  • Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)
    Anti-Cullin 2/CUL-2 antibody [EPR3104(2)] - BSA and Azide free (ab240149)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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