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Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR11912(B)] to IGFBP7 - BSA and Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, WB
  • Reacts with: Human

Overview

  • Product name

    Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free
    See all IGFBP7 primary antibodies

  • Description

    Rabbit monoclonal [EPR11912(B)] to IGFBP7 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • SW480, 293T and MCF7 cell lysates; Human pancreas and prostate tissues; MCF7 cells; 293T cells.

  • General notes

    Ab202765 is the carrier-free version of ab171085. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab202765 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Western blot - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Western blot - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    All lanes : Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/1000 dilution

    Lane 1 : SW480 cell lysate
    Lane 2 : 293T cell lysate
    Lane 3 : MCF7 cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 29 kDa



    This data was developed using ab171085, the same antibody clone in a different buffer formulation.

    This image was produced using unpurified antibody.

  • Immunocytochemistry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Immunocytochemistry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)

    This data was developed using ab171085, the same antibody clone in a different buffer formulation.

    Immunocytochemistry/ Immunofluorescence analysis of MCF7 (human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with Purified ab171085 at 1:100 (6.0 ?g/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 μg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 μg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow Cytometry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Flow Cytometry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)

    This data was developed using ab171085, the same antibody clone in a different buffer formulation.

    Flow Cytometry analysis of MCF7 (Human breast adenocarcinoma epithelial cell) cells labeling IGFBP7 with purified ab171085 at 1:60 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue).
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)

    This data was developed using ab171085, the same antibody clone in a different buffer formulation.

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lung cancer tissue sections labeling IGFBP7 with purified ab171085 at 1:250 dilution (2.4 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use). PBS instead of the primary antibody was used as the negative control.
  • Western blot - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Western blot - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Anti-IGFBP7 antibody [EPR11912(B)] (ab171085) at 1/10000 dilution (Purified) + SW480 (Human colorectal adenocarcinoma epithelial cell) whole cell lysates at 15 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Predicted band size: 29 kDa
    Observed band size: 29 kDa



    This data was developed using ab171085, the same antibody clone in a different buffer formulation.

  • Flow Cytometry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Flow Cytometry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    This data was developed using ab171085, the same antibody clone in a different buffer formulation.Flow cytometric analysis of permeabilized 293T cells labeling IGFBP7 with ab171085 at 1/250 dilution (red) compared to a rabbit IgG negative control (green). This image was produced using unpurified antibody.
  • Immunocytochemistry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Immunocytochemistry - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    This data was developed using ab171085, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of MCF7 cells labeling IGFBP7 with ab171085 at 1/250 dilution. This image was produced using unpurified antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    This data was developed using ab171085, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human prostate tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    This data was developed using ab171085, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling IGFBP7 with ab171085 at 1/50 dilution. This image was produced using unpurified antibody.
  • Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)
    Anti-IGFBP7 antibody [EPR11912(B)] - BSA and Azide free (ab202765)

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