Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
![Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)](https://www.abcam.com/ps/products/231/ab231706/Images/ab231706-320709-anti-rab8a-phospho-t72-antibody-mjf-r20-dot-blot.jpg "Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [MJF-R20] to RAB8A (phospho T72) - BSA and Azide free
- Suitable for: WB, Dot blot
- Reacts with: Mouse, Human
Overview
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Product name
Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free
See all RAB8A primary antibodies -
Description
Rabbit monoclonal [MJF-R20] to RAB8A (phospho T72) - BSA and Azide free -
Host species
Rabbit -
Specificity
ab230260 cross-reacts with phosphorylated Rab3A, Rab10, Rab35 and Rab43. Please note that the immunogen sequence was derived from Rab8A but is identical to that of Rab8B. -
Tested Applications & Species
See all applications and species dataApplication Species Dot RatWB Mouse
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A, Rab10 and Rab35 expression vectors, treated with 150 nM MLi-2.
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General notes
ab231706 is the carrier-free version of ab230260 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab231706 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Please see PMID: 29127256 . Lis P et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J 475:1-22 (2018).
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
MJF-R20 -
Isotype
IgG -
Research areas
Images
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Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
Dot Blot - Anti-Rab8A (phospho T72) antibody [MJF-R20] (ab230260) used at a 1/1000 dilution.
Lane 1: Rab8 (phospho T72) peptide.
Lane 2: Rab8 non-phospho peptide.
ab97051 secondary antibody used at a 1/100,000 dilution.
Exposure time: 32 seconds.
Blocking/Dilution buffer and concentration: 5% NFDM/TBST.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230260).
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![Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)](https://www.abcam.com/ps/products/231/ab231706/Images/ab231706-302432-anti-rab8a-phospho-t72-antibody-mjf-r20-western-blot.jpg)
Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)All lanes : Anti-RAB8A (phospho T72) antibody [MJF-R20] (ab230260) at 1/1000 dilution
Lane 1 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 2 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 3 : HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab10 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 4 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Lane 5 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab43 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
Secondary
All lanes : IRDye 800CW secondary antibody at 1/25000 dilution
Predicted band size: 24 kDaBlocking buffer: 5% NFDM/TBST.
Dilution buffer: 5% BSA/TBST.
The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates Rab8 phosphorylation in MEF (mouse embryonic fibroblasts).
The results show that this antibody recognizes other LRRK2-phosphorylated Rab proteins (Rab3A, Rab10, and Rab35). The phospho-threonine site in Rab8 (T72) differs from that of Rab3A (T86); Rab10 (T73); and Rab43 (T82).
The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID: 29127256).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230260).
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![Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)](https://www.abcam.com/ps/products/231/ab231706/Images/ab231706-3-benefits-of-recombinant-antibodies.png)
Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
