Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [MJF-R20] to RAB8A (phospho T72) - BSA and Azide free
  • Suitable for: WB, Dot blot
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free
    See all RAB8A primary antibodies

  • Description

    Rabbit monoclonal [MJF-R20] to RAB8A (phospho T72) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab230260 cross-reacts with phosphorylated Rab3A, Rab10, Rab35 and Rab43. Please note that the immunogen sequence was derived from Rab8A but is identical to that of Rab8B.

  • Tested Applications & Species

    Application Species
    Dot
    Rat
    WB
    Mouse
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A, Rab10 and Rab35 expression vectors, treated with 150 nM MLi-2.

  • General notes

    ab231706 is the carrier-free version of ab230260 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    Ab231706 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Please see PMID: 29127256 . Lis P et al. Development of phospho-specific Rab protein antibodies to monitor in vivo activity of the LRRK2 Parkinson's disease kinase. Biochem J 475:1-22 (2018).

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
    Dot Blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)

    Dot Blot - Anti-Rab8A (phospho T72) antibody [MJF-R20] (ab230260) used at a 1/1000 dilution.

    Lane 1: Rab8 (phospho T72) peptide.

    Lane 2: Rab8 non-phospho peptide.

    ab97051 secondary antibody used at a 1/100,000 dilution.

    Exposure time: 32 seconds.

    Blocking/Dilution buffer and concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230260).

  • Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
    Western blot - Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
    All lanes : Anti-RAB8A (phospho T72) antibody [MJF-R20] (ab230260) at 1/1000 dilution

    Lane 1 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab3A expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
    Lane 2 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab8A expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
    Lane 3 : HEK-293 cells transfected with LRRK2[Y1699C] and HA-tagged Rab10 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
    Lane 4 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab35 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng
    Lane 5 : HEK-293 cells transfected with LRRK2 [Y1699C] and HA-tagged Rab43 expression vectors, were treated with150 nM MLi-2 for 90 minutes, whole cell lysate, 100 ng

    Secondary
    All lanes : IRDye 800CW secondary antibody at 1/25000 dilution

    Predicted band size: 24 kDa



    Blocking buffer: 5% NFDM/TBST.

    Dilution buffer: 5% BSA/TBST.

    The LRRK2 pathogenic mutation Y1699C increases LRRK2 activity and markedly elevates Rab8 phosphorylation in MEF (mouse embryonic fibroblasts).

    The results show that this antibody recognizes other LRRK2-phosphorylated Rab proteins (Rab3A, Rab10, and Rab35). The phospho-threonine site in Rab8 (T72) differs from that of Rab3A (T86); Rab10 (T73); and Rab43 (T82).

    The images were kindly provided by our collaborator, Dr. Dario Alessi, and have been published (PMID: 29127256).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab230260).

  • Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)
    Anti-RAB8A (phospho T72) antibody [MJF-R20] - BSA and Azide free (ab231706)

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