Antibodies, Proteins, Kits and Reagents for Life Science

311 587 ₸ Product size

100 µl 311 587 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB8A antibody [EPR14873] (ab188574)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR14873] to RAB8A
Suitable for: WB, IHC-P, ICC/IF, Flow Cyt
Knockout validated
Reacts with: Mouse, Rat, Human

Product name

Anti-RAB8A antibody [EPR14873]
See all RAB8A primary antibodies
Description

Rabbit monoclonal [EPR14873] to RAB8A
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: HepG2, HeLa, 293T, HCT-116, C6, Raw264.7, PC-12 and NIH3T3 lysates and human brain tissue lysate. IHC-P: Human stomach and human adenocarcinoma of colon tissues. ICC/IF: HepG2 cells. Flow Cyt: HeLa cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR14873
Isotype

IgG
Research areas

Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)

All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RAB8A knockout HeLa cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : Human brain tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Lanes 1-4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab188574 Anti-RAB8A antibody [EPR14873] was shown to specifically react with RAB8A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264993 (knockout cell lysate ab257195) was used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)

All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution

Lane 1 : Wild-type HeLa cell lysate
Lane 2 : RAB8A knockout HeLa cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : Human brain tissue lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 24 kDa
Observed band size: 24 kDa

Lanes 1-4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, ab8245 observed at 37 kDa.

ab188574 Anti-RAB8A antibody [EPR14873] was shown to specifically react with RAB8A in wild-type HeLa cells. Loss of signal was observed when knockout cell line ab264994 (knockout cell lysate ab257196) was used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 10000 dilution for 1 hour at room temperature before imaging.
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)

All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution

Lane 1 : Wild-type HAP1 cell lysate
Lane 2 : RAB8A knockout HAP1 cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : NIH/3T3 cell lysate

Lysates/proteins at 20 µg per lane.

Predicted band size: 24 kDa

Lanes 1 - 4: Merged signal (red and green). Green - ab188574 observed at 24 kDa. Red - loading control, ab8245, observed at 37 kDa.

ab188574 was shown to specifically react with RAB8A when RAB8A knockout samples were used. Wild-type and RAB8A knockout samples were subjected to SDS-PAGE. ab188574 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)

Immunohistochemical analysis of paraffin-embedded Human adenocarcinoma of colon tissue labelling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)

All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution

Lane 1 : C6 lysate
Lane 2 : RAW264.7 lysate
Lane 3 : PC-12 lysate
Lane 4 : NIH3T3 lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)

Anti-RAB8A antibody [EPR14873] (ab188574) at 1/1000 dilution + HCT-116 lysate at 20 µg

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated

Predicted band size: 24 kDa
Observed band size: 24 kDa
Western blot - Anti-RAB8A antibody [EPR14873] (ab188574)

All lanes : Anti-RAB8A antibody [EPR14873] (ab188574) at 1/10000 dilution

Lane 1 : HepG2 lysate
Lane 2 : HeLa lysate
Lane 3 : 293T lysate

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 24 kDa
Observed band size: 24 kDa
Flow Cytometry - Anti-RAB8A antibody [EPR14873] (ab188574)

Flow Cytometrical analysis of HeLa cells labeling RAB8A with ab188574 at 1/260 (pink) compared to a Isotype control (green). FITC-conjugated goat-anti-rabbit secondary antibody at 1/150 were used for the analysis.
Immunocytochemistry/ Immunofluorescence - Anti-RAB8A antibody [EPR14873] (ab188574)

Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labelling RAB8A with ab188574 at 1/500 followed by Goat anti rabbit IgG (Alexa Fluor^® 488) and DAPI staining.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB8A antibody [EPR14873] (ab188574)

Immunohistochemical analysis of paraffin-embedded Human stomach tissue labeling RAB8A with ab188574 at 1/500. A prediluted HRP Polymer for Rabbit IgG was used as the secondary antibody. Counter stained with Hematoxylin.

Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Anti-RAB8A antibody [EPR14873] (ab188574)

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