Antibodies, Proteins, Kits and Reagents for Life Science

53 606 ₸ Product size

10 µl 53 606 ₸ 100 µl 154 118 ₸

Order now and get it on Tuesday March 02, 2021

Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [MJF-R27-30] to RAB8A (phospho S111)
Suitable for: WB
Knockout validated
Reacts with: Human

Product name

Anti-RAB8A (phospho S111) antibody [MJF-R27-30]
See all RAB8A primary antibodies
Description

Rabbit monoclonal [MJF-R27-30] to RAB8A (phospho S111)
Host species

Rabbit
Tested Applications & Species

Application Species
WB
Human

See all applications and species data
Immunogen

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
Positive control
- WB: Wild-type HeLa (treated with 10 uM CCCP), whole cell lysate.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Application	Species
WB	Human

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

MJF-R27-30
Isotype

IgG
Research areas

Western blot - Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492)

All lanes : Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492) at 1/1000 dilution

Lane 1 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO
Lane 2 : Wild-type HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP
Lane 3 : RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with DMSO
Lane 4 : RAB8A knockout HeLa (human cervix adenocarcinoma epithelial cell) treated with 10 uM CCCP

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100000 dilution

Predicted band size: 24 kDa
Observed band size: 23 kDa
why is the actual band size different from the predicted?

Blocking and diluting buffer and concentration: 5% NFDM/TBST.

CCCP (carbonyl cyanide m-chlorophenyl hydrazone) inhibits oxidative phosphorylation.

The lysates were kindly provided by Pawan Singh Ph.D, University of Dundee.

Exposure time: 3min
Anti-RAB8A (phospho S111) antibody [MJF-R27-30] (ab267492)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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