Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR17321] to RAB5C/RABL
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

Overview

  • Product name

    Anti-RAB5C/RABL antibody [EPR17321]
    See all RAB5C/RABL primary antibodies

  • Description

    Rabbit monoclonal [EPR17321] to RAB5C/RABL

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: HEK293, HepG2 and U937 cell lysates; Human fetal heart lysate, IHC-P: Human kidney tissue. ICC/IF: HepG2 and MCF7 cells,

  • General notes

     This product was previously labelled as RAB5C

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    All lanes : Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/1000 dilution

    Lane 1 : HEK293 (Human embryonic kidney) cell lysate
    Lane 2 : HepG2 (Human liver hepatocellular carcinoma) cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 10 seconds


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunocytochemistry/ Immunofluorescence - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Immunocytochemistry/ Immunofluorescence - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

    Ab199530 staining RAB5C/RABL in HepG2 cells (Human hepatocellular carcinoma epithelial cell) by Immunocytochemistry/Immunofluorescence (ICC/IF). Cells were fixed with 100% Methanol. Samples were incubated with primary antibody at 1:500 dilution (2.2µg/ml). An AlexaFluor®488 Goat anti-Rabbit (ab150077) was used as a secondary antibody at 1:1000 dilution (2µg/ml). An Anti-Alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594), ab195889 was used as a counterstain antibody at 1:200 dilution (2.5µg/ml). DAPI was used as a nuclear counterstain. Confocal image showing cytoplasmic staining in HepG2 cells.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

    Immunohistochemical analysis of paraffin-embedded Human kidney tissue labeling RAB5C/RABL with ab199530 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) secondary antibody at 1/500 dilution. Cytoplasm staining of Human kidney tissue is observed. Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody and secondary antibody Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/10000 dilution + U937 (Human histiocytic lymphoma cells) cell lysate at 20 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Western blot - Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Anti-RAB5C/RABL antibody [EPR17321] (ab199530) at 1/1000 dilution + Human fetal heart tissue lysate at 10 µg

    Secondary
    Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 23 kDa
    Observed band size: 23 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Anti-RAB5C/RABL antibody [EPR17321] (ab199530)
    Anti-RAB5C/RABL antibody [EPR17321] (ab199530)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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