Astana Biomed Group, an authorized Abcam distributor in Central Asia

Recombinant Human RHEB protein (BSA and azide free) (ab173075)

Key features and details

  • Expression system: Escherichia coli
  • Purity: > 95% SDS-PAGE
  • Endotoxin level:
  • Tags: GST tag N-Terminus
  • Suitable for: SDS-PAGE, HPLC

Description

  • Product name

    Recombinant Human RHEB protein (BSA and azide free)
    See all RHEB proteins and peptides

  • Purity

    > 95 % SDS-PAGE.
    Greater than 95% as determined by SEC-HPLC and reducing SDS-PAGE.

  • Endotoxin level

  • Expression system

    Escherichia coli

  • Accession

    Q15382

  • Protein length

    Full length protein

  • Animal free

    No

  • Carrier free

    Yes

  • Nature

    Recombinant

    • Species

      Human

    • Sequence

      MPQSKSRKIAILGYRSVGKSSLTIQFVEGQFVDSYDPTIENTFTKLITVN GQEYHLQLVDTAGQDEYSIFPQTYSIDINGYILVYSVTSIKSFEVIKVIH GKLLDMVGKVQIPIMLVGNKKDLHMERVISYEEGKALAESWNAAFLESSA KENQTAVDVFRRIILEAEKMDGAASQGKSSCSVM

    • Predicted molecular weight

      20 kDa

    • Amino acids

      1 to 184

    • Tags

      GST tag N-Terminus

    • Specifications

    Our Abpromise guarantee covers the use of ab173075 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      SDS-PAGE

      HPLC

    • Form

      Liquid
    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

      pH: 8.00
      Constituents: 0.24% Tris, 0.31% Glutathione

    General Info

    • Alternative names

      • Ras homolog enriched in brain 2, formerly
      • GTP binding protein Rheb
      • GTP-binding protein Rheb
      • MGC111559
      • Ras homolog enriched in brain
      • Ras homolog enriched in brain 2
      • Rheb
      • RHEB 2
      • RHEB_HUMAN
      • RHEB2
      • RHEB2, formerly
      see all

    • Function

      Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.

    • Tissue specificity

      Ubiquitous. Highest levels observed in skeletal and cardiac muscle.

    • Sequence similarities

      Belongs to the small GTPase superfamily. Rheb family.

    • Post-translational
      modifications

      Farnesylation is important for efficiently activating mTORC1-mediated signaling.

    • Cellular localization

      Cell membrane.
    • Information by UniProt

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (0)

Publishing research using ab173075? Please let us know so that we can cite the reference in this datasheet.

ab173075 has not yet been referenced specifically in any publications.

Preparation and Storage

  • Stability and Storage

    Shipped on Dry Ice. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    pH: 8.00
    Constituents: 0.24% Tris, 0.31% Glutathione

Images

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