Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CSL4 antibody [EPR13525] (ab181167)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR13525] to CSL4
  • Suitable for: WB, IHC-P, ICC/IF, Flow Cyt, IP
  • Reacts with: Human

Overview

  • Product name

    Anti-CSL4 antibody [EPR13525]
    See all CSL4 primary antibodies

  • Description

    Rabbit monoclonal [EPR13525] to CSL4

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • 293T, Jurkat or HepG2 lysate, Human breast carcinoma tissue, HepG2 or K562 cells.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR13525

  • Isotype

    IgG

  • Research areas

Images

  • Western blot - Anti-CSL4 antibody [EPR13525] (ab181167)
    Western blot - Anti-CSL4 antibody [EPR13525] (ab181167)
    All lanes : Anti-CSL4 antibody [EPR13525] (ab181167) at 1/2000 dilution

    Lane 1 : 293T lysate
    Lane 2 : Jurkat lysate
    Lane 3 : HepG2 lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 21 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13525] (ab181167)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CSL4 antibody [EPR13525] (ab181167)

    Immunohistochemical analysis of paraffin embedded Human breast carcinoma tissue labeling CSL4 with ab181167 at a 1/100 dilution. Prediluted HRP Polymer for Rabbit IgG secondary used. Counterstained with Hematoxylin.

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Immunoprecipitation - Anti-CSL4 antibody [EPR13525] (ab181167)
    Immunoprecipitation - Anti-CSL4 antibody [EPR13525] (ab181167)

    Detection of Human CSL4 by Western Blot of Immunoprecipitate. 293T whole cell lysate (lane 1) and a negative control (lane 2) loaded, ab181167 used for blotting immunoprecipitated CSL4 at a 1/50 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG secondary used at a 1/1500 dilution.

  • Flow Cytometry - Anti-CSL4 antibody [EPR13525] (ab181167)
    Flow Cytometry - Anti-CSL4 antibody [EPR13525] (ab181167)

    Flow cytometry analysis of K562 cells using ab181167 at a 1/90 dilution (red) or a Rabbit monoclonal IgG (negative) (green). Goat anti rabbit IgG (FITC) secondary used at a 1/150 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13525] (ab181167)
    Immunocytochemistry/ Immunofluorescence - Anti-CSL4 antibody [EPR13525] (ab181167)

    Immunofluorescence analysis of HepG2 cells (fixative 4% paraformaldehyde) labeling CSL4 with ab181167 at a 1/250 dilution. Goat anti rabbit IgG (Alexa Fluor® 555) secondary used at a 1/200 diution.

  • Anti-CSL4 antibody [EPR13525] (ab181167)
    Anti-CSL4 antibody [EPR13525] (ab181167)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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