Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-TIM44 antibody [EPR16821] (ab194829)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR16821] to TIM44
  • Suitable for: WB, ICC/IF, IP, IHC-P
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-TIM44 antibody [EPR16821]
    See all TIM44 primary antibodies

  • Description

    Rabbit monoclonal [EPR16821] to TIM44

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • A431, HepG2 and K562 cell lysates; Mouse heart lysate; Human endometrial adenocarcinoma tissue; A431 cells.

  • General notes

     

     

    Previously labelled as TIMM44. 

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR16821

  • Isotype

    IgG

  • Research areas

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (ab194829)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TIM44 antibody [EPR16821] (ab194829)

    Immunohistochemical analysis of paraffin-embedded Human endometrial adenocarcinoma tissue labeling TIM44 with ab194829 at 1/100 dilution (5μg/ml). A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.
    Inset image: negative control obtained using PBS instead of ab194829. Cytoplasm staining on humanendometrial adenocarcinoma was observed.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Western blot - Anti-TIM44 antibody [EPR16821] (ab194829)
    Western blot - Anti-TIM44 antibody [EPR16821] (ab194829)
    Anti-TIM44 antibody [EPR16821] (ab194829) at 1/1000 dilution + mouse heart lysate at 10 µg

    Secondary
    Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (ab194829)
    Immunocytochemistry/ Immunofluorescence - Anti-TIM44 antibody [EPR16821] (ab194829)

    Immunofluorescent analysis of A431 cells labeling TIM44 with ab194829 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on A431 cell line was observed.

  • Western blot - Anti-TIM44 antibody [EPR16821] (ab194829)
    Western blot - Anti-TIM44 antibody [EPR16821] (ab194829)
    All lanes : Anti-TIM44 antibody [EPR16821] (ab194829) at 1/5000 dilution

    Lane 1 : A431 cell lysate
    Lane 2 : HepG2 cell lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

    Predicted band size: 51 kDa

  • Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (ab194829)
    Immunoprecipitation - Anti-TIM44 antibody [EPR16821] (ab194829)

    Immunoprecipitation analysis of immunoprecipitation pellet from K562 cell lysate immunoprecipitated using ab194826 at 1/20 dilution (Lane 1).  Lane 2: PBS instead of K562 cell lysate. ab194829 at 1/1000 was used for subsequent western blot detection. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 was used as secondary antibody.

  • Anti-TIM44 antibody [EPR16821] (ab194829)
    Anti-TIM44 antibody [EPR16821] (ab194829)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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