Anti-ENO1 antibody [253] - N-terminal (ab190365)
![Anti-ENO1 antibody [253] - N-terminal (ab190365)](https://www.abcam.com/ps/products/190/ab190365/Images/ab190365-225915-anti-eno1-antibody-253-n-terminal-immunofluorescence.jpg "Anti-ENO1 antibody [253] - N-terminal (ab190365)")
Key features and details
- Mouse monoclonal [253] to ENO1 - N-terminal
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG1
Overview
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Product name
Anti-ENO1 antibody [253] - N-terminal
See all ENO1 primary antibodies -
Description
Mouse monoclonal [253] to ENO1 - N-terminal -
Host species
Mouse -
Specificity
ab190365 was tested for binding to expressed bovine ENO1, ENO2 and ENO3 and shown to be specific for only ENO1. -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Pig
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Immunogen
Synthetic peptide corresponding to Cow ENO1 (N terminal). Immunogen corresponds to 12 amino acids synthesized on a 8-amine lysine core using the multiple antigen presentation method.
Database link: Q9XSJ4 -
Positive control
- HeLa cell lysate and rat 3T3 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.07% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
253 -
Isotype
IgG1 -
Research areas
Images
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Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [253] - N-terminal (ab190365)
Immunofluorescence analysis of rat 3T3 cells, labeling ENO1 using ab190365 at a 1/2000 dilution (green). Counterstained with an antibody against Vimentin (red) and DNA (blue).
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![Western blot - Anti-ENO1 antibody [253] - N-terminal (ab190365)](https://www.abcam.com/ps/products/190/ab190365/Images/ab190365-225916-anti-eno1-antibody-253-n-terminal-western-blot.jpg)
Western blot - Anti-ENO1 antibody [253] - N-terminal (ab190365)Anti-ENO1 antibody [253] - N-terminal (ab190365) at 1/5000 dilution + HeLa (Human epithelial cell line from cervix adenocarcinoma) cell lysate
Predicted band size: 47 kDa
Observed band size: 47 kDa
