Antibodies, Proteins, Kits and Reagents for Life Science

335 040 ₸ Product size

100 µl 335 040 ₸

Order now and get it on Tuesday March 02, 2021

Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR10863(B)] to ENO1
Suitable for: Flow Cyt, WB, ICC/IF, IP
Reacts with: Mouse, Rat, Human

Product name

Anti-ENO1 antibody [EPR10863(B)]
See all ENO1 primary antibodies
Description

Rabbit monoclonal [EPR10863(B)] to ENO1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Synthetic peptide within Human ENO1 aa 1-100 (N terminal). The exact sequence is proprietary. Synthetic peptide within Human ENO1 aa 1-50
Database link: P06733
(Peptide available as ab226769)
Positive control
- Purchase matching WB positive control:Recombinant human ENO1 protein (Active)
- WB: MCF7, Jurkat, A431, C2C12, NIH/3T3 and HeLa whole cell lysate (ab150035), mouse heart and rat brain tissue lysate. ICC/IF: MCF7 cells. IP: HeLa whole cell lysate (ab150035).
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
Storage buffer

Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR10863(B)
Isotype

IgG
Research areas

Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified) + Rat brain tissue lysate at 10 µg

Secondary
HRP-conjugated goat anti-rabbit IgG at 1/1000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor^® 594-conjugated goat anti-mouse IgG (1/1000).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000).
Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)

Lane 1 : C2C12 whole cell lysate
Lane 2 : NIH/3T3 whole cell lysate
Lane 3 : Mouse heart tissue lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 with unpurified ab155102 at a dilution of 1/100.
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/5000 dilution (purified)

Lane 1 : MCF-7 whole cell lysate
Lane 2 : Jurkat whole cell lysate
Lane 3 : HeLa whole cell lysate
Lane 4 : A431 whole cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilution

Predicted band size: 47 kDa
Observed band size: 47 kDa

Blocking and dilution buffer: 5% NFDM/TBST.
Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.
Lane 1 (input): HeLa whole cell lysate (10µg)
Lane 2 (+): ab155102 + HeLa whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.
For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).
Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-ENO1 antibody [EPR10863(B)] (ab155102)

All lanes : Anti-ENO1 antibody [EPR10863(B)] (ab155102) at 1/1000 dilution (unpurified)

Lane 1 : MCF7 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : A431 cell lysate
Lane 4 : HeLa cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

Predicted band size: 47 kDa
Anti-ENO1 antibody [EPR10863(B)] (ab155102)

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