Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19602] to ATP6V1E1
  • Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ATP6V1E1 antibody [EPR19602]
    See all ATP6V1E1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19602] to ATP6V1E1

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Rat
    Human
    IHC-P
    Human
    IP
    Human
    WB
    Mouse
    Rat
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A-673, 293, HeLa, HepG2 and RAW 264.7 whole cell lysates; Human kidney, liver and brain lysates; Mouse brain and kidney lysates; Rat brain lysate. IHC-P: Human testis and glioma tissues. ICC/IF: HepG2 and C6 cells. Flow Cyt: HeLa cells. IP: HepG2 whole cell lysate.

  • General notes

     

     

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Images

  • Western blot - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Western blot - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    All lanes : Anti-ATP6V1E1 antibody [EPR19602] (ab201468) at 1/1000 dilution

    Lane 1 : A-673 (Human muscle Ewing's Sarcoma cell line) whole cell lysate
    Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
    Lane 3 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 4 : HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1: 1 minute; Lane 2: 30 seconds; Lane 3/4: 3 minutes.

  • Western blot - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Western blot - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    All lanes : Anti-ATP6V1E1 antibody [EPR19602] (ab201468) at 1/1000 dilution

    Lane 1 : Human kidney lysate
    Lane 2 : Human liver lysate
    Lane 3 : Human brain lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa



    Blocking/Dilution buffer: 5% NFDM/TBST.

    Exposure time: Lane 1/3: 3 minutes; Lane 2: 1 minute.

  • Western blot - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Western blot - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    All lanes : Anti-ATP6V1E1 antibody [EPR19602] (ab201468) at 1/1000 dilution

    Lane 1 : Mouse brain lysate
    Lane 2 : Mouse kidney lysate
    Lane 3 : Rat brain lysate
    Lane 4 : RAW 264.7 (Mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution

    Predicted band size: 26 kDa
    Observed band size: 26 kDa


    Exposure time: 3 minutes


    Blocking/Dilution buffer: 5% NFDM/TBST.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling ATP6V1E1 with ab201468 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on human testis is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

    Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling ATP6V1E1 with ab201468 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Cytoplasm staining on human glioma is observed.

    Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.

    Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HepG2 (Human liver hepatocellular carcinoma cell line) cells labeling ATP6V1E1 with ab201468 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on HepG2 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab201468 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Immunocytochemistry/ Immunofluorescence - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

    Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized C6 (Rat glial tumor cell line) cells labeling ATP6V1E1 with ab201468 at 1/100 dilution, followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic staining on C6 cell line. The nuclear counterstain is DAPI (blue).

    Tubulin is detected with Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution and Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution (red).

    The negative controls are as follows:-

    -ve control 1: ab201468 at 1/100 dilution followed by Goat Anti-Mouse IgG H&L (Alexa Fluor® 594) (ab150120) secondary antibody at 1/1000 dilution.

    -ve control 2: Anti-alpha Tubulin mouse MAb (ab7291) at 1/1000 dilution followed by Goat Anti-Rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

  • Flow Cytometry - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Flow Cytometry - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

    Flow cytometric analysis of 4% paraformaldehyde-fixed HeLa (Human epithelial cells from cervix adenocarcinoma) cells labeling ATP6V1E1 with ab201468 at 1/60 dilution (red) compared with a rabbit monoclonal IgG isotype control (ab172730; black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody; blue). Goat anti rabbit IgG (Alexa Fluor® 488) at 1/500 dilution was used as the secondary antibody.

  • Immunoprecipitation - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Immunoprecipitation - Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

    ATP6V1E1 was immunoprecipitated from 0.35mg of HepG2 (Human liver hepatocellular carcinoma cell line) whole cell lysate with ab201468 at 1/40 dilution.

    Western blot was performed from the immunoprecipitate using ab201468 at 1/500 dilution.

    VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

    Lane 1: HepG2 whole cell lysate 10µg (Input).

    Lane 2: ab201468 IP in HepG2 whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab201468 in HepG2 whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

  • Anti-ATP6V1E1 antibody [EPR19602] (ab201468)
    Anti-ATP6V1E1 antibody [EPR19602] (ab201468)

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