Anti-ENO1 antibody [2G2AG11BF8] (ab112994)
![Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](https://www.abcam.com/ps/products/112/ab112994/Images/ab112994-125709-anti-eno1-antibody-2g2ag11bf8-immunoprecipitation.jpg "Anti-ENO1 antibody [2G2AG11BF8] (ab112994)")
Key features and details
- Mouse monoclonal [2G2AG11BF8] to ENO1
- Suitable for: IP, Flow Cyt, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-ENO1 antibody [2G2AG11BF8]
See all ENO1 primary antibodies -
Description
Mouse monoclonal [2G2AG11BF8] to ENO1 -
Host species
Mouse -
Tested applications
Suitable for: IP, Flow Cyt, ICC/IFmore details -
Species reactivity
Reacts with: Human
Does not react with: Non human primates -
Immunogen
Tissue, cells or virus. This information is considered to be commercially sensitive.
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Positive control
- HeLa and HL60 cells; HeLa, Human Liver and Heart homogenates.
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General notes
This antibody clone is manufactured by Abcam.
Product was previously marketed under the MitoSciences sub-brand.
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.5
Preservative: 0.02% Sodium azide
Constituent: 99.98% HEPES buffered saline -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
ab112994 was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Monoclonal purity was near homogeneity as judged by SDS-PAGE (>95%). -
Clonality
Monoclonal -
Clone number
2G2AG11BF8 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Immunoprecipitation - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)Immunprecipitation of ENO1 by ab112994.
Staining of ENO1 (47 kDa) from HeLa cell lysate (1), Human liver (2) and Heart (3) homogenates. The identity of the protein was confirmed by mass spectrometry. This gel was stained with colloidal Coomassie blue G. -
![Flow Cytometry - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](https://www.abcam.com/ps/products/112/ab112994/Images/ab112994-125708-anti-eno1-antibody-2g2ag11bf8-flow-cytometry.jpg)
Flow Cytometry - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)ab112994 at 1ug/ml staining ENO1 in MeOH-fixed HL60 cells by Flow Cytometry(blue). Isotype control antibody (red). -
![Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)](https://www.abcam.com/ps/products/112/ab112994/Images/ab112994-125707-anti-eno1-antibody-2g2ag11bf8-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [2G2AG11BF8] (ab112994)ab112994 at 5ug/ml staining ENO1 in HeLa cells by Immunocytochemistry (4% paraformaldehyde fixed and 0.1% Triton X-100 permeabilized) followed by Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1 hour (green). The target locates mainly to the cytoplasm.
