Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR10863(B)] to ENO1 - BSA and Azide free
  • Suitable for: ICC, IP, Flow Cyt, WB
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free
    See all ENO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR10863(B)] to ENO1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IP, Flow Cyt, WBmore details
    Unsuitable for: IHC-P

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • MCF7, Jurkat, A431 and HeLa whole cell lysate (ab150035); MCF7 cells.

  • General notes

    Ab206120 is the carrier-free version of ab155102. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab206120 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Images

  • Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Immunoprecipitation - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

    ab155102 (purified) at 1/20 immunoprecipitating ENO1 in HeLa whole cell lysate.

    Lane 1 (input): HeLa whole cell lysate (10µg)

    Lane 2 (+): ab155102 + HeLa whole cell lysate.

    Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab155102 in HeLa whole cell lysate.

    For western blotting, a HRP-conjugated anti-rabbit IgG, specific to the non-reduced form of IgG was used as the secondary antibody (1/1500).

    Blocking buffer and concentration: 5% NFDM/TBST.

    Diluting buffer and concentration: 5% NFDM /TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

  • Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Flow Cytometry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

    Flow Cytometry analysis of MCF-7 (human breast carcinoma) cells labeling ENO1 with purified ab155102 at 1/20 dilution(10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) were used as the unlabeled control.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

  • Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

    Immunocytochemistry/Immunofluorescence analysis of MCF-7 cells labelling ENO1 with purified ab155102 at 1/60. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000) were also used.

    Control 1: primary antibody (1/60) and secondary antibody, ab150120, an Alexa Fluor® 594-conjugated goat anti-mouse IgG (1/1000).

    Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

  • Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

    Clone EPR10863(B) (ab206120) has been successfully conjugated by Abcam. This image was generated using Anti-ENO1 antibody [EPR10863(B)] (Alexa Fluor® 647). Please refer to ab205872 for protocol details.

    ab205872 staining ENO1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205872 at a 1/250 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at a 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

    Clone EPR10863(B) (ab206120) has been successfully conjugated by Abcam. This image was generated using Anti-ENO1 antibody [EPR10863(B)] (Alexa Fluor® 488). Please refer to ab205871 for protocol details.

    ab205871 staining ENO1 in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab205871 at a 1/250 dilution (shown in green) and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at a 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

  • Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Immunocytochemistry - Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

    Immunocytochemistry/Immunofluorescence analysis of MCF7 cells labelling ENO1 with unpurified ab155102 at a dilution of 1/100.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab155102).

  • Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)
    Anti-ENO1 antibody [EPR10863(B)] - BSA and Azide free (ab206120)

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