Galactose Assay Kit (ab83382)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric/Fluorometric
- Platform: Microplate reader
- Assay time: 40 min
- Sample type: Cell culture supernatant, Other biological fluids, Plasma, Serum, Tissue Extracts, Urine
- Sensitivity: 10 nmol/well
Overview
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Product name
Galactose Assay Kit -
Detection method
Colorimetric/Fluorometric -
Sample type
Cell culture supernatant, Urine, Serum, Plasma, Other biological fluids, Tissue Extracts -
Assay type
Quantitative -
Sensitivity
Assay time
0h 40mProduct overview
Galactose Assay Kit (Colorimetric/Fluorometric) (ab83382) provides a simple and convenient method for direct measurement of galactose levels in various biological samples such as serum, plasma and other body fluids, as well as food and growth media. In this assay, oxidation of galactose generates a product that reacts with the galactose probe to produce color (OD570nm) or fluorescence (Ex/Em 535/587 nm). Liquid samples can be tested directly without purification. The assay is fast, convenient and sensitive. It can also be used as a high throughput assay.
Visit our FAQs page for tips and troubleshooting.Notes
Galactose (C6H12O6 MW: 180.16 g/mol) is one of the major naturally occurring sugars, a monosaccharide that differs from glucose only by the configuration of the hydroxyl group at the carbon-4 position. Often present as an anomeric mixture of α-D-galactose and β-D-galactose, this monosaccharide exists abundantly in milk, dairy products and many other food types such as fruits and vegetables.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.Platform
Microplate readerProperties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Galactose Assay Buffer WM, 1 x 25ml Galactose Enzyme Mix (Lyophilized) Green 1 vial Galactose Probe (DMSO solution) Red 1 x 0.2ml Galactose Standard (100 nmol/ul) Yellow 1 x 100µl HRP (Lyophilized) Purple 1 vial -
Research areas
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Relevance
Galactose (C6H12O6 FW: 180.16) is one the major naturally occurring sugars, involved in many biological processes.
Properties
-
Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 100 tests Galactose Assay Buffer WM, 1 x 25ml Galactose Enzyme Mix (Lyophilized) Green 1 vial Galactose Probe (DMSO solution) Red 1 x 0.2ml Galactose Standard (100 nmol/ul) Yellow 1 x 100µl HRP (Lyophilized) Purple 1 vial -
Research areas
-
Relevance
Galactose (C6H12O6 FW: 180.16) is one the major naturally occurring sugars, involved in many biological processes.
Images
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Functional Studies - Galactose Assay Kit (ab83382)
Galactose measured in tissue lysates showing quantity (nmol) per mg of extracted protein.
Protein concentration for samples varied from 7 mg/mL to 17 mg/mL. Samples were tested undiluted.
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Functional Studies - Galactose Assay Kit (ab83382)Galactose measured in cell lysates showing quantity (nmol) per 1 mln cells.
Samples with the concentration of 4e6 cells/mL were used. Samples were tested undiluted.
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Functional Studies - Galactose Assay Kit (ab83382)Fluorometric standard curve: mean of duplicates (+/- SD) with background reads subtracted.
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Functional Studies - Galactose Assay Kit (ab83382)Galactose levels measured in biological fluids showing quantity (nmol) per mL. Samples tested undiluted.
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Functional Studies - Galactose Assay Kit (ab83382)Example of Galactose standard curve obtained with ab83382
