Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19758] to ENO1 - BSA and Azide free
  • Suitable for: WB, ICC/IF, IP, Flow Cyt, IHC-P
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-ENO1 antibody [EPR19758] - BSA and Azide free
    See all ENO1 primary antibodies

  • Description

    Rabbit monoclonal [EPR19758] to ENO1 - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    ab229378 does not cross-react with ENO2 or ENO3.

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Mouse
    IHC-P
    Mouse
    IP
    Human
    See all applications and species data

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human pancreas tissue.

  • General notes

    Ab229378 is the carrier-free version of ab227978. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab229378 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Immunohistochemical analysis of paraffin-embedded rat pancreas tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in medium-small ducts of rat pancreas is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Immunoprecipitation - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunoprecipitation - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    ENO1 was immunoprecipitated from 0.35 mg of Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate with ab227978 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227978 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: Jurkat whole cell lysate 10 μg (Input).

    Lane 2: ab227978 IP in Jurkat whole cell lysate.

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227978 in Jurkat whole cell lysate.

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 3 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Flow Cytometry - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Flow Cytometry - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 with ab227978 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue).

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in medium-small ducts of mouse pancreas is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Immunohistochemical analysis of paraffin-embedded human clear cell renal cell carcinoma tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Membranous and nuclear staining in human clear cell kidney carcinoma (PMID: 26037892) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Immunofluorescent analysis of 4% paraformaldegyde-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryo fibroblast cell line) cells labeling ENO1 with ab227978 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and nuclear staining in NIH/3T3 cell line.

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    -ve control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunocytochemistry/ Immunofluorescence - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Immunofluorescent analysis of 4% paraformaldegyde-fixed, 0.1% Triton X-100 permeabilized HeLa (human epithelial cell line from cervix adenocarcinoma) cells labeling ENO1 with ab227978 at 1/500 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and weakly nuclear staining in HeLa cell line. 

    The nuclear counterstain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).

    -ve control: PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution .

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue labeling ENO1 with ab227978 at 1/2000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Cytoplasmic and nuclear staining in medium-small ducts of human pancreas (PMID: 19425054) is observed. Counter stained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227978).

  • Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)
    Anti-ENO1 antibody [EPR19758] - BSA and Azide free (ab229378)

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