Antibodies, Proteins, Kits and Reagents for Life Science

304 886 ₸ Product size

100 µl 304 886 ₸ 10 µl 120 614 ₸

Order now and get it on Tuesday March 02, 2021

Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPNCIR129] to TXNRD1
Suitable for: WB, IP, IHC-P, Flow Cyt, ICC/IF
Reacts with: Mouse, Rat, Human

Product name

Anti-TXNRD1 antibody [EPNCIR129]
See all TXNRD1 primary antibodies
Description

Rabbit monoclonal [EPNCIR129] to TXNRD1
Host species

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
ICC/IF	Human
IHC-P	Human
IP	Human
WB	Mouse Rat Human

See all applications and species data

Immunogen

Recombinant full length protein corresponding to Mouse TXNRD1.
Database link: Q9JMH6
Positive control
- Mouse liver, NIH 3T3, RAW264.7, Human fetal liver and Rat liver lysates; HeLa cells.
General notes
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Dolph Hatfield. View antibodies from NCI Center for Cancer Research Collaboration.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 0.05% BSA, 59% PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPNCIR129
Isotype

IgG
Research areas

Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

All lanes : Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution (purified)

Lane 1 : rat liver lysate
Lane 2 : NIH/3T3 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : HRP goat anti-rabbit IgG (H+L) at 1/20000 dilution

Predicted band size: 55, 67 kDa
Observed band size: 55 kDa
why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution (purified) + HeLa cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 55, 67 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/10000 dilution (purified) + HepG2 cell lysate at 10 µg

Secondary
HRP goat anti-rabbit IgG (H+L) at 1/100000 dilution

Predicted band size: 55, 67 kDa
Observed band size: 55 kDa why is the actual band size different from the predicted?

Blocking buffer: 5% NFDM/TBST
Dilution buffer: 5% NFDM/TBST
Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Immunofluorescence staining of HeLa cells with purified ab124954 at a working dilution of 1/150, counter-stained with DAPI. The secondary antibody was Alexa Fluor^® 488 goat anti-rabbit (ab150077), used at a dilution of 1/1000. ab7291, a mouse anti-tubulin antibody (1/1000), was used to stain tubulin along with ab150120 (Alexa Fluor^® 594 goat anti-mouse, 1/1000), shown in the top right hand panel. The cells were fixed in 4% PFA and permeabilized using 0.1% Triton X 100. The negative controls are shown in bottom middle and right hand panels - for negative control 1, purified ab124954 was used at a dilution of 1/500 followed by an Alexa Fluor^® 594 goat anti-mouse antibody (ab150120) at a dilution of 1/500. For negative control 2, ab7291 (mouse anti-tubulin) was used at a dilution of 1/500 followed by an Alexa Fluor^® 488 goat anti-rabbit antibody (ab150077) at a dilution of 1/400.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Immunohistochemical staining of paraffin embedded human testis with purified ab124954 at a working dilution of 1/150. The secondary antibody used is HRP goat anti-rabbit IgG H&L (ab97051) at 1/500. The sample is counter-stained with hematoxylin. Antigen retrieval was perfomed using Tris-EDTA buffer, pH 9.0. PBS was used instead of the primary antibody as the negative control, and is shown in the inset.
Immunoprecipitation - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

ab124954 (purified) at 1/40 immunoprecipitating TXNRD1 in 10 μg Jurkat (Lanes 1 and 2, observed at 55 kDa). Lane 3 - PBS. For western blotting, VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10,000 dilution. Blocking buffer and concentration: 5% NFDM/TBST Dilution buffer and concentration: 5% NFDM/TBST
Flow Cytometry - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Overlay histogram showing HeLa cells fixed in 4% PFA and stained with purified ab124954 at a dilution of 1 in 120 (red line). The secondary antibody used was FITC goat anti-rabbit at a dilution of 1 in 500. Rabbit monoclonal IgG was used as an isotype control (black line) and cells incubated in the absence of both primary and secondary antibody were used as a negative control (blue line).
Western blot - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

All lanes : Anti-TXNRD1 antibody [EPNCIR129] (ab124954) at 1/1000 dilution (unpurified)

Lane 1 : Mouse liver lysates
Lane 2 : NIH 3T3 lysates
Lane 3 : RAW264.7 lysates
Lane 4 : Human fetal liver lysates
Lane 5 : Rat liver lysates

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : Goat anti-Rabbit HRP at 1/2000 dilution

Predicted band size: 55, 67 kDa
Immunocytochemistry/ Immunofluorescence - Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Unpurified ab124954, at 1/100 dilution, staining TXNRD1 in HeLa cells by Immunofluorescence.
Anti-TXNRD1 antibody [EPNCIR129] (ab124954)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com