ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.

Lane 1: HeLa cell lysates.

Lane 2: 293T cell lysates.

Lane 3: Jurkat cell lysates.

ab16840 at 1/2000 dilution staining human TXNRD1 in HeLa, Jurkat, and 293T lysates by Western blot.

Lane 1: HeLa cell lysates.

Lane 2: 293T cell lysates.

Lane 3: Jurkat cell lysates.

ab16840 staining TXNRD1 in human mammary tissue by Immunohistochemistry (paraffin embedded sections).
Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab16840 at 1/400 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each Reagent

ab16840 (2µg/ml) staining TXNRD1 in human breast using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear and cytoplasmic staining of acini.
Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffer citrate pH 6.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.