Human BST2 Matched Antibody Pair Kit (ab221442)
Key features and details
- Unlabeled capture antibody, biotin-labeled detection antibody and calibrated protein standard
- For economical ELISA and ELISA-based assay development
- Reacts with: Human
- Range: 7.8 pg/ml - 500 pg/ml
Overview
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Product name
Human BST2 Matched Antibody Pair Kit
See all BST2/Tetherin kits -
Detection method
Colorimetric -
Assay type
ELISA set -
Sensitivity
1.2 pg/ml -
Range
7.8 pg/ml - 500 pg/ml -
Species reactivity
Reacts with: Human -
Product overview
Human BST2 Matched Antibody Pair Kits include a capture and a biotinylated detector antibody pair, along with a calibrated protein standard, suitable for sandwich ELISA. The Matched Antibody Pair Kit can be used to quantify native and recombinant human BST2.
Optimization of the kit reagents to sample type, immunoassay format or instrumentation may be required. Guidelines for use of this kit in a standard 96-well microplate sandwich ELISA using HRP/TMB system of colorimetric detection is described in this assay procedure for the purposes of quantification.
Protocol information and tips on the use of the Matched Antibody Pair kits for sandwich ELISA can be found on our website. An accessory pack can be purchased which includes buffer reagents required to perform 10 x 96-well plate sandwich ELISAs (ab210905).
For additional information on the performance of the antibody pair used in this kit, please see our equivalent SimpleStep ELISA kit ab231931. Please note that while the antibody pair is the same provided in the corresponding SimpleStep ELISA Kit, due to differences in their formulation, this antibody pair cannot be used with the consumables provided with our SimpleStep ELISA Kits.
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Tested applications
Suitable for: ELISAmore details -
Platform
Reagents
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 10 x 96 tests 5 x 96 tests Human BST2 Capture Antibody 2 x 50µg 1 x 50µg Human BST2 Detector Antibody 2 x 12.5µg 1 x 12.5µg Human BST2 Lyophilized Protein 2 vials 1 vial -
Research areas
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Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
Sequence similarities
Belongs to the tetherin family. -
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
Post-translational
modificationsMonoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
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Alternative names
- Bone marrow stromal antigen 2
- Bone marrow stromal cell antigen
- Bone marrow stromal cell antigen 2
see all -
Database links
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
Images
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Human BST2 standard curve
Standard calibration curve. Background substracted values are graphed.
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Sandwich ELISA - Human BST2 Matched Antibody Pair Kit (ab221442)To learn more about the advantages of recombinant antibodies see here.
