Human BST2 ELISA Kit (ab231931)
Key features and details
- One-wash 90 minute protocol
- Sensitivity: 0.28 pg/ml
- Range: 5 pg/ml - 300 pg/ml
- Sample type: Cell culture extracts, Cell culture supernatant, Cit plasma, EDTA Plasma, Hep Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
-
Product name
Human BST2 ELISA Kit
See all BST2/Tetherin kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Serum 9 2% Inter-assay Sample n Mean SD CV% Serum 3 8.2% -
Sample type
Cell culture supernatant, Serum, Cell culture extracts, Hep Plasma, EDTA Plasma, Cit plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
0.28 pg/ml -
Range
5 pg/ml - 300 pg/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 101 99% - 103% Cell culture extracts 113 109% - 117% Cell culture media 100 99% - 100% Hep Plasma 96 93% - 1010% EDTA Plasma 102 99% - 104% Cit plasma 100 98% - 103% -
Assay time
1h 30m -
Assay duration
One step assay -
Species reactivity
Reacts with: Human
Does not react with: Cow -
Product overview
Human BST2 ELISA Kit (ab231931) is a single-wash 90 min sandwich ELISA designed for the quantitative measurement of BST2 protein in cell culture extracts, cell culture supernatant, cit plasma, edta plasma, hep plasma, and serum. It uses our proprietary SimpleStep ELISA® technology. Quantitate Human BST2 with NULL NULL sensitivity.
SimpleStep ELISA® technology employs capture antibodies conjugated to an affinity tag that is recognized by the monoclonal antibody used to coat our SimpleStep ELISA® plates. This approach to sandwich ELISA allows the formation of the antibody-analyte sandwich complex in a single step, significantly reducing assay time. See the SimpleStep ELISA® protocol summary in the image section for further details. Our SimpleStep ELISA® technology provides several benefits:
- Single-wash protocol reduces assay time to 90 minutes or less
- High sensitivity, specificity and reproducibility from superior antibodies
- Fully validated in biological samples
- 96-wells plate breakable into 12 x 8 wells stripsA 384-well SimpleStep ELISA® microplate (ab203359) is available to use as an alternative to the 96-well microplate provided with SimpleStep ELISA® kits.
-
Notes
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses. -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
-
Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Human BST2 Capture Antibody 1 x 600µl 10X Human BST2 Detector Antibody 1 x 600µl 10X Wash Buffer PT (ab206977) 1 x 20ml 5X Cell Extraction Buffer PTR (ab193970) 1 x 10ml Antibody Diluent 5BR 1 x 6ml Human BST2 Lyophilized Recombinant Protein 2 vials Plate Seals 1 unit Sample Diluent NS (ab193972) 1 x 12ml SimpleStep Pre-Coated 96-Well Microplate (ab206978) 1 unit Stop Solution 1 x 12ml TMB Development Solution 1 x 12ml -
Research areas
-
Function
May be involved in the sorting of secreted proteins (By similarity). May be involved in pre-B-cell growth. Antiretroviral defense protein, that blocks release of retrovirus from the cell surface. Depleted unpon HIV-1 infection by viral VPU protein through 20S proteasome degradation. Depleted upon infection by human Kaposi's sarcoma-associated herpesvirus (KSHV) through ubiquitination and subsequent degradation. May play a role in B-cell activation in rheumatoid arthritis. -
Tissue specificity
Predominantly expressed in liver, lung, heart and placenta. Lower levels in pancreas, kidney, skeletal muscle and brain. Overexpressed in multiple myeloma cells. Highly expressed during B-cell development, from pro-B precursors to plasma cells. Highly expressed on T-cells, monocytes, NK cells and dendritic cells (at protein level). -
Sequence similarities
Belongs to the tetherin family. -
Domain
The extracellular coiled coil domain is important for virus retention at the cell surface and prevention of virus spreading. -
Post-translational
modificationsMonoubiquitinated by KSHV E3 ubiquitin-protein ligase K5, leading to its targeting to late endosomes and degradation. -
Cellular localization
Golgi apparatus > trans-Golgi network. Cell membrane. Cell membrane. Late endosome. Targeted to late endosomes upon KSHV infection and subsequent ubiquitination. Targeted to the trans-Golgi network by viral VPU protein. - Information by UniProt
-
Alternative names
- Bone marrow stromal antigen 2
- Bone marrow stromal cell antigen
- Bone marrow stromal cell antigen 2
see all -
Database links
- Entrez Gene: 684 Human
- Omim: 600534 Human
- SwissProt: Q10589 Human
- Unigene: 118110 Human
Images
-
Other - Human BST2 ELISA Kit (ab231931)
SimpleStep ELISA technology allows the formation of the antibody-antigen complex in one single step, reducing assay time to 90 minutes. Add samples or standards and antibody mix to wells all at once, incubate, wash, and add your final substrate. See protocol for a detailed step-by-step guide.
-
Example of human BST2 standard curve in 1X Cell Extraction Buffer PTR.The BST2 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
-
Interpolated concentrations of native BST2 in human serum and plasma samples.The concentrations of BST2 were measured in duplicates, interpolated from the BST2 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 100%, plasma (heparin) 100%, plasma (EDTA) 50%, and plasma (citrate) 12.5%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration in the neat samples was determined to be 172 pg/mL in serum, 148 pg/mL in plasma (heparin), 133 pg/mL in plasma (EDTA), and 206 pg/mL in plasma (citrate).
-
Interpolated concentrations of spiked BST2 in RPMI media and of native BST2 in cell extract samples.The concentrations of BST2 were measured in duplicate and interpolated from the BST2 standard curve and corrected for sample dilution. Undiluted samples are as follows: RPMI media 50%, A431 cell extract 10 µg/mL, and HeLa cell extract 30 µg/mL. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).
-
Serum from ten individual healthy human female donors was measured in duplicate.Interpolated dilution factor corrected values are plotted (mean +/- SD, n=2). The mean BST2 concentration was determined to be 221 pg/mL with a range of 74 – 672 pg/mL.
-
Example of human BST2 standard curve in Sample Diluent NS.The BST2 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.
-
Sandwich ELISA - Human BST2 ELISA Kit (ab231931)To learn more about the advantages of recombinant antibodies see here.
