Anti-VAV2 antibody (ab86699)
Key features and details
- Rabbit polyclonal to VAV2
- Suitable for: WB, ICC/IF
- Reacts with: Rabbit, Human
- Isotype: IgG
Overview
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Product name
Anti-VAV2 antibody
See all VAV2 primary antibodies -
Description
Rabbit polyclonal to VAV2 -
Host species
Rabbit -
Specificity
ab86699 detects VAV2 in human A431 cells and detects several VAV isoforms in Jurkat cells. The immunogen sequence has significant homology to the conserved site in VAV1 and VAV3. -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF RabbitWB Human
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Immunogen
Synthetic peptide:
EDEGDDIYED
conjugated to KLH, corresponding to amino acids 165-174 of Mouse VAV2.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 50% Glycerol, PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-VAV2 antibody (ab86699)All lanes : Anti-VAV2 antibody (ab86699) at 1/1000 dilution
Lane 1 : A431 cells treated with EGF
Lane 2 : A431 cells treated with EGF, exposed to alkaline
phosphatase
Predicted band size: 100 kDa
Observed band size: 100 kDa
Additional bands at:
Western blot membranes were incubated with diluted antibody in 5% non-fat milk, PBS, 0.04% Tween20 for 1hour at room temperature. -
Immunocytochemistry/ Immunofluorescence - Anti-VAV2 antibody (ab86699)Immunocytochemical labeling of VAV2 in paraformaldehyde-fixed and NP40 permeabilized rabbit spleen fibroblasts. The cells were labeled with ab86699 at a 1/50 dilution and detected using an appropriate secondary antibody conjugated to Cy3.
