Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1067Y] to VAV2 - BSA and Azide free
  • Suitable for: IP, IHC-P, Flow Cyt, WB
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-VAV2 antibody [EP1067Y] - BSA and Azide free
    See all VAV2 primary antibodies

  • Description

    Rabbit monoclonal [EP1067Y] to VAV2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IP, IHC-P, Flow Cyt, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human cervical carcinoma. WB: HeLa, HAP1, 293 cell lysates. Flow Cyt: HeLa cells. IP: HEK293 cell lysate.

  • General notes

    Ab239846 is the carrier-free version of ab52640. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab239846 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Western blot - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    Western blot - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    All lanes : Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution

    Lane 1 : Wild-type HeLa cell lysate
    Lane 2 : VAV2 knockout HeLa cell lysate

    Lysates/proteins at 20 µg per lane.

    Performed under reducing conditions.

    Predicted band size: 101 kDa
    Observed band size: 100 kDa
    why is the actual band size different from the predicted?



    This data was developed using the same antibody clone in a different buffer formulation (ab52640).

      Lanes 1- 2: Merged signal (red and green). Green - ab52640 observed at 100 kDa. Red - Anti-GAPDH antibody [6C5] - Loading Control (ab8245) observed at 37 kDa.

     ab52640 was shown to react with VAV2 in wild-type HeLa cells in western blot. Loss of signal was observed when knockout cell line ab265318 (knockout cell lysate ab257794) was used. Wild-type HeLa and VAV2 knockout HeLa cell lysates were subjected to SDS-PAGE. Membrane was blocked for 1 hour at room temperature in 0.1% TBST with 3% non-fat dried milk. ab52640 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) overnight at 4°C at a 1 in 20000 dilution and a 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye®800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye®680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)

    Human cervical carcinoma stained with ab52640 at 1/50 - 1/100 dilution

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52640).

    Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

  • Western blot - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    Western blot - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    All lanes : Anti-VAV2 antibody [EP1067Y] (ab52640) at 1/20000 dilution

    Lane 1 : Wild-type HAP1 whole cell lysate
    Lane 2 : VAV2 knockout HAP1 whole cell lysate

    Lysates/proteins at 20 µg per lane.

    Predicted band size: 101 kDa



    This data was developed using the same antibody clone in a different buffer formulation (ab52640).

    Lanes 1 - 4: Merged signal (red and green). Green - ab52640 observed at 101 kDa. Red - loading control, ab9484, observed at 37 kDa.

    ab52640 was shown to specifically react with VAV2 in wild-type HAP1 cells as signal was lost in VAV2 knockout cells. Wild-type and VAV2 knockout samples were subjected to SDS-PAGE. Ab52640 and ab9484 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/20000 dilution and 1/20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.

  • Flow Cytometry - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    Flow Cytometry - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)

    Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling VAV2 (red) with ab52640 at a 1/2500 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52640).

  • Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    Immunoprecipitation - Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    VAV2 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 10µg of Rabbit monoclonal to VAV2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
    The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
    Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab52640.
    Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
    Band: 100kDa; VAV2.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab52640).

  • Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)
    Anti-VAV2 antibody [EP1067Y] - BSA and Azide free (ab239846)

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