Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR21017-123] to MSH2 - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, IP, Flow Cyt
  • Knockout validated
  • Reacts with: Human

Overview

  • Product name

    Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free
    See all MSH2 primary antibodies

  • Description

    Rabbit monoclonal [EPR21017-123] to MSH2 - BSA and Azide free

  • Host species

    Rabbit

  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Human
    IP
    Human
    See all applications and species data

  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human testis tissue.

  • General notes

    Ab228334 is the carrier-free version of ab227941. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab228334 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue labeling MSH2 with ab227941 at 1/8000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in para-carcinoma colonic epithelium (image B) or stromal cells (both image A and B) and loss of expression in the paired colon cancer (image A) (PMID: 24710284). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    Immunofluorescent analysis of 100% methanol-fixed A-375 (human malignant melanoma cell line) cells labeling MSH2 with ab227941 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in A-375 cell line is shown.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    Immunofluorescent analysis of 100% methanol-fixed A549 (human lung carcinoma cell line) cells labeling MSH2 with ab227941 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Nuclear staining in A549 cell line is shown.

    The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) (red) at 1/200 dilution.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Flow Cytometry - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Flow Cytometry - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized A-375 (human malignant melanoma cell line) cell line labeling MSH2 with ab227941 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabeled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/2000 dilution was used as the secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Immunoprecipitation - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Immunoprecipitation - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    MSH2 was immunoprecipitated from 0.35 mg of A-375 (human malignant melanoma cell line) lysate with ab227941 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab227941 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

    Lane 1: A-375 whole cell lysate 10 µg (Input). 

    Lane 2: ab227941 IP in A-375 whole cell lysate. 

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab227941 in A-375 whole cell lysate.

    Exposure time: 1 second.

    Blocking and dilution buffer concentration: 5% NFDM/TBST.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Flow Cytometry - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Flow Cytometry - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    Overlay histogram showing HAP1 wildtype (green line) and HAP1-MSH2 knockout cells (red line) stained with ab227941. The cells were fixed with 80% methanol (5 min) (left panel) or 4% formaldehyde (10 min) (right panel) , and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by ab227941 for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) presorbed (ab150081) at 1/2000 dilution for 30 min at 22°C.

    A rabbit IgG isotype control antibody  (ab172730) was used at the same concentration and conditions as the primary antibody (HAP1 wildtype - black line, HAP1-MSH2  knockout - grey line). Unlabelled sample was also used as a control (this line is not shown for the purpose of simplicity).

    Acquisition of >5,000 events were collected using a 50 mW Blue laser (488nm) and 530/30 bandpass filter.

    Note: We recommend fixing cells using MeOH instead of PFA to get optimal results.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

    Immunohistochemical analysis of paraffin-embedded human testis tissue labeling MSH2 with ab227941 at 1/8000 dilution, followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP). Nuclear staining in human testis was observed (PMID: 10029069). Counter stained with Hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP).

    Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227941).

  • Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)
    Anti-MSH2 antibody [EPR21017-123] - BSA and Azide free (ab228334)

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