HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)
![HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)](https://www.abcam.com/ps/products/198/ab198491/Images/ab198491-257504-WBab1984911.jpg "HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)")
Key features and details
- HRP Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine
- Suitable for: WB
- Reacts with: Cow, Human
- Conjugation: HRP
- Isotype: IgG2b
Overview
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Product name
HRP Anti-3-Nitrotyrosine antibody [7A12AF6]
See all 3-Nitrotyrosine primary antibodies -
Description
HRP Mouse monoclonal [7A12AF6] to 3-Nitrotyrosine -
Host species
Mouse -
Conjugation
HRP -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Cow, Human
Predicted to work with: Mouse, Rat
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Immunogen
Chemical/ Small Molecule corresponding to 3-Nitrotyrosine. Nitrated KLH (Keyhole Limpet Hemocyanin).
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Positive control
- WB: Nitrated liver homogenate and nitrated bovine serum albumin.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 30% Glycerol (glycerin, glycerine), 1% BSA, PBS -
Concentration information loading... -
Purity
Proprietary Purification -
Purification notes
The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Purity >95% by SDS-PAGE. -
Clonality
Monoclonal -
Clone number
7A12AF6 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Images
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Western blot - HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491)All lanes : HRP Anti-3-Nitrotyrosine antibody [7A12AF6] (ab198491) at 1/5000 dilution
Lane 1 : Human liver tissue lysate - total protein (ab29889)
Lane 2 : Nitrated Human Liver Homogenate Standard (ab131380)
Lane 3 : Bovine Serum Albumin
Lane 4 :Bovine Serum Albumin (nitrated ) (ab131379)
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 secondThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab198491 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
