Anti-ITPA antibody [EPR8779] (ab150420)
![Anti-ITPA antibody [EPR8779] (ab150420)](https://www.abcam.com/ps/products/150/ab150420/Images/ab150420-397674-anti-itpa-antibody-epr8779-western-blot-human-knockout-lysate.jpg "Anti-ITPA antibody [EPR8779] (ab150420)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8779] to ITPA
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
-
Product name
Anti-ITPA antibody [EPR8779]
See all ITPA primary antibodies -
Description
Rabbit monoclonal [EPR8779] to ITPA -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
-
Immunogen
Synthetic peptide within Human ITPA aa 1-100 (internal sequence). The exact sequence is proprietary.
-
Positive control
- WB: HEK-293T, Jurkat, HepG2, HeLa and K562 cell lysates.
-
General notes
This product was previously labelled as Inosine triphosphate pyrophosphatase
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20ºC. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR8779 -
Isotype
IgG -
Research areas
Images
-
Western blot - Anti-ITPA antibody [EPR8779] (ab150420)All lanes : Anti-ITPA antibody [EPR8779] (ab150420) at 1/1000 dilution
Lane 1 : Wild-type HEK293T cell lysate
Lane 2 : ITPA knockout HEK293T cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) at 1/10000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDaLanes 1-3: Merged signal (red and green). Green - ab150420 observed at 21 kDa. Red - loading control ab8245 observed at 36 kDa.
ab150420 Anti-ITPA antibody [EPR8779] was shown to specifically react with ITPA in wild-type HEK293T cells. Loss of signal was observed when knockout cell line ab266679 (knockout cell lysate ab258477) was used. Wild-type and ITPA knockout samples were subjected to SDS-PAGE. ab150420 and Anti-GAPDH antibody [6C5] - Loading Control (ab8245) were incubated overnight at 4°C at 1 in 1000 dilution and 1 in 20000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1 in 20000 dilution for 1 hour at room temperature before imaging.
-
![Western blot - Anti-ITPA antibody [EPR8779] (ab150420)](https://www.abcam.com/ps/products/150/ab150420/Images/ab150420-157071-anti-inosine-triphosphate-pyrophosphatase-antibody-epr8779-western-blot.jpg)
Western blot - Anti-ITPA antibody [EPR8779] (ab150420)All lanes : Anti-ITPA antibody [EPR8779] (ab150420) at 1/1000 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : K562 cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 21 kDa
-
![Anti-ITPA antibody [EPR8779] (ab150420)](https://www.abcam.com/ps/products/150/ab150420/Images/ab150420-4-benefits-of-recombinant-antibodies.png)
Anti-ITPA antibody [EPR8779] (ab150420)
