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Anti-3-Nitrotyrosine antibody [39B6] (ab61392)

Key features and details

  • Mouse monoclonal [39B6] to 3-Nitrotyrosine
  • Suitable for: IHC-Fr, WB
  • Isotype: IgG2a

Overview

  • Product name

    Anti-3-Nitrotyrosine antibody [39B6]
    See all 3-Nitrotyrosine primary antibodies

  • Description

    Mouse monoclonal [39B6] to 3-Nitrotyrosine

  • Host species

    Mouse

  • Specificity

    No detectable crossreactivity with non-nitrated tyrosine. Not species specific.

  • Tested applications

    Suitable for: IHC-Fr, WBmore details

  • Immunogen

    3-(4-Hydroxy-3-nitrophenyl acetamido) propionic acid-BSA conjugate.

  • Positive control

    • SIN-1 (3-morpholinosydnonimine) treated BSA. Use between 1-2.5mM SIN-1 for 1-24 hours, grow cells under normal growth conditions. Please see Yasuda S, Idell S, Liu MC. Biochem J. 2007 Jan 15;401(2):497-503. PMID: 17002600. IHC-P: Mouse back skin and inflammed colon tissue; Human colon carcinoma tissue. Rat liver tissue. WB: A549 cell lysate.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

Properties

Images

  • Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)

    ab61392 staining 3-nitrotyrosine in Bouin's fixed paraffin-embedded Rat liver tissue at 1/1000 dilution. FITC-conjugated goat anti-mouse IgG(green) was used as a secondary antibody.

  • Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)

    ab61392 staining 3-nitrotyrosine in Bouin's fixed paraffin-embedded backskin sections of transgenic mice.

    Blocker: 5% goat serum in buffer. Buffer: 99% washing buffer (7g BSA and 222ul gelatin topped up to 1L dH2O), 1% heat inactivated goat serum. Primary Dilution: 1/100 for 1 hour at room temperature. Secondary: FITC-conjugated goat anti-mouse IgG, 1/50 for 1 hour at room temperature.

  • Western blot - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Western blot - Anti-3-Nitrotyrosine antibody [39B6] (ab61392) This image is courtesy of an anonymous Abreview
    All lanes : Anti-3-Nitrotyrosine antibody [39B6] (ab61392) at 1/3000 dilution

    All lanes : Mouse skeletal muscle

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat anti-Mouse HRP conjugated at 1/6000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 4 seconds


    Blocked with 5% milk for 2 hours.

    See Abreview

  • Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)

    Formalin-fixed, paraffin-embedded mouse inflammed colon tissue stained for Nitrotyrosine using ab61392 at 1/1000000 dilution in immunohistochemical analysis.

    Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.

  • Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Immunohistochemistry (Frozen sections) - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)

    Formalin-fixed, paraffin-embedded human colon carcinoma tissue stained for Nitrotyrosine using ab61392 at 1/25000 dilution in immunohistochemical analysis.

    Secondary Antibody: Biotin Goat Anti-Mouse at 1/2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.

  • Western blot - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Western blot - Anti-3-Nitrotyrosine antibody [39B6] (ab61392)
    Anti-3-Nitrotyrosine antibody [39B6] (ab61392) at 1/1000 dilution (2.5 hours at RT with shaking) + A549 (Human lung carcinoma cell line) cell lysate at 15 µg

    Secondary
    Goat anti-mouse IgG HRP, 1 hour at RT with shaking at 1/1000 dilution


    Block: 5% Skim milk powder in TBST.

    Color Development: Chemiluminescent for HRP for 5 mins at RT.

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