Anti-MSH2 antibody [EPR3944] (ab133330)
![Anti-MSH2 antibody [EPR3944] (ab133330)](https://www.abcam.com/ps/products/133/ab133330/Images/ab133330-256357-anti-msh2-antibody-epr3944-western-blot.jpg "Anti-MSH2 antibody [EPR3944] (ab133330)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3944] to MSH2
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MSH2 antibody [EPR3944]
See all MSH2 primary antibodies -
Description
Rabbit monoclonal [EPR3944] to MSH2 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide within Human MSH2 aa 900-1000. The exact sequence is proprietary.
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Positive control
- K562, HeLa, and A375 cell lysates.
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General notes
To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading... -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR3944 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MSH2 antibody [EPR3944] (ab133330)
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MSH2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A375 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab133330 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab133330 was shown to recognize MSH2 when MSH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. ab133330 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Anti-MSH2 antibody [EPR3944] (ab133330)](https://www.abcam.com/ps/products/133/ab133330/Images/ab133330-3-benefits-of-recombinant-antibodies.png)
Anti-MSH2 antibody [EPR3944] (ab133330)
