Anti-MSH2 antibody (ab70270)
Key features and details
- Rabbit polyclonal to MSH2
- Suitable for: ICC/IF, WB, IP, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-MSH2 antibody
See all MSH2 primary antibodies -
Description
Rabbit polyclonal to MSH2 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Guinea pig, Cow, Pig, Rhesus monkey, Gorilla, African green monkey, Common marmoset, Orangutan, Elephant
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Immunogen
Synthetic peptide: LEREQGEKII QEFLSKVKQM PFTEMSEENI TIKLKQLKAE VIAKNNSFVN EIISRIKVTT , corresponding to C terminal amino acids 875-934 of Human MSH2
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Positive control
- HeLa whole cell lysate (ab150035) Ramos whole cell lysate NIH3T3 whole cell lysate
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General notes
To see more of the key markers and tools you need to study the hallmarks of cancer, including genome instability and mutation, please visit the following page.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MSH2 antibody (ab70270)All lanes : Anti-MSH2 antibody (ab70270) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : Ramos whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 105 kDa
Observed band size: 116 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MSH2 antibody (ab70270)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human metastatic lymph node (left) and mouse squamous cell carcinoma (right) tissues labelling MSH2 with ab70270 at 1/1000 (1µg/ml) and 1/5000 (0.2µg/ml). Detection: DAB. -
Immunoprecipitation - Anti-MSH2 antibody (ab70270)Immunoprecipitation of HeLa whole cell lysate. Lane 1: 50µg of input lysate. Lane 2: HeLa whole cell lysate (1mg) immunoprecipitated with ab70270 at 3µg/mg. Lane 3: HeLa whole cell lysate immunoprecipitated with control IgG. Samples were subjected to Western blot, analysed with ab70270 at 0.1µg/ml and detected by chemiluminescence with an exposure time of 3 minutes. -
Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (ab70270) This image is courtesy of an Abreview submitted by Kirk McManusab70270 staining MSH2 in HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.5% Triton X-100 in PBS. Samples were incubated with primary antibody (1/500 in PBS) for 1 hour at 22°C. ab150081, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Counterstained with DAPI.
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Immunocytochemistry/ Immunofluorescence - Anti-MSH2 antibody (ab70270)ICC/IF image of ab70270 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab70270, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
