Anti-MSH2 antibody [EPR3943] - BSA and Azide free (ab214456)
![Anti-MSH2 antibody [EPR3943] - BSA and Azide free (ab214456)](https://www.abcam.com/ps/products/214/ab214456/Images/ab214456-303329-anti-msh2-antibody-epr3943-western-blot.jpg "Anti-MSH2 antibody [EPR3943] - BSA and Azide free (ab214456)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR3943] to MSH2 - BSA and Azide free
- Suitable for: WB
- Knockout validated
- Reacts with: Human
Overview
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Product name
Anti-MSH2 antibody [EPR3943] - BSA and Azide free
See all MSH2 primary antibodies -
Description
Rabbit monoclonal [EPR3943] to MSH2 - BSA and Azide free -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: A375, A431, SW480 and HeLa cell lysates IHC-P: Human colonic adenocarcinoma ICC/IF: HeLa cells
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General notes
ab214456 is the carrier-free version of ab92473 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with
Ab214456 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR3943 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-MSH2 antibody [EPR3943] - BSA and Azide free (ab214456)
This WB data was generated using the same anti-MSH2 antibody clone, EPR3943, in a different buffer formulation (cat# ab92473).
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: MSH2 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: A375 cell lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab92473 observed at 115 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab92473 was shown to recognize MSH2 when MSH2 knockout samples were used, along with additional cross-reactive bands. Wild-type and MSH2 knockout samples were subjected to SDS-PAGE. ab92473 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging. -
![Anti-MSH2 antibody [EPR3943] - BSA and Azide free (ab214456)](https://www.abcam.com/ps/products/214/ab214456/Images/ab214456-3-benefits-of-recombinant-antibodies.png)
Anti-MSH2 antibody [EPR3943] - BSA and Azide free (ab214456)
