Astana Biomed Group, an authorized Abcam distributor in Central Asia

Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [CAL38] to CD8 alpha - BSA and Azide free
  • Suitable for: IHC-P, IHC-Fr, IP
  • Reacts with: Mouse

Overview

  • Product name

    Anti-CD8 alpha antibody [CAL38] - BSA and Azide free
    See all CD8 alpha primary antibodies

  • Description

    Rabbit monoclonal [CAL38] to CD8 alpha - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IHC-Fr, IPmore details
    Unsuitable for: Flow Cyt

  • Species reactivity

    Reacts with: Mouse

  • Immunogen

    Synthetic peptide. This information is considered to be commercially sensitive.

  • Positive control

    • IHC-P: Mouse spleen tissue. IHC-Fr: Mouse Thymus tissue. IP: Mouse thymus tissue lysate.

  • General notes

    Ab251609 is the carrier-free version of ab237723. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with

    ab251609 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

Properties

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)

    Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling CD8 alpha with ab237723 at 1/500 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    Positive staining on the mouse spleen is shown. The section was incubated with ab237723 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237723).

  • Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)
    Immunohistochemistry (Frozen sections) - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)

    Immunohistochemical analysis of 4% paraformaldehyde-fixed, 0.2% Triton X-100 permeabilized frozen mouse thymus tissue labeling CD8 alpha with ab237723 at at 1/250 dilution, follwed by a AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution. Positive staining on the membrane of T cells in mouse thymus (PMID: 29042640) is observed. Counterstained with DAPI (Blue).

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a AlexaFluor®488 Goat anti-Rabbit secondary antibody (ab150077) at 1/1000 dilution.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237723).

  • Immunoprecipitation - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)
    Immunoprecipitation - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)

    CD8 alpha was immunoprecipitated from 0.35 mg of Mouse thymus tissue lysate with ab237723 at 1/20 dilution. Western blot was performed on the immunoprecipitate using ab237723 at at 1/1000 dilution. VeriBlot for IP secondary antibody (HRP) (ab131366) was used as the secondary antibody at 1/5000 dilution.

    Lane 1: Mouse thymus tissue lysate 10µg (input)
    Lane 2: ab237723 IP in mouse thymus tissue lysate.
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab237723 in Mouse thymus lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 30 seconds.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237723).

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)

    Formalin-fixed, paraffin-embedded mouse spleen tissue stained for CD8 alpha with ab237723 at 0.125 µg/ml in immunohistochemical analysis.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab237723).

  • Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)
    Anti-CD8 alpha antibody [CAL38] - BSA and Azide free (ab251609)

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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