Anti-DCTN1/p150-glued antibody (ab96004)
Key features and details
- Rabbit polyclonal to DCTN1/p150-glued
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DCTN1/p150-glued antibody
See all DCTN1/p150-glued primary antibodies -
Description
Rabbit polyclonal to DCTN1/p150-glued -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1216-1248.
Database link: NP_004073 -
Positive control
- Jurkat whole cell lysate (ab7899) for WB. HeLa cells for IF. OV CA for IHC-P.
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General notes
Previously labelled as DCTN1.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min
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All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution
Lane 1 : Jurkat cell lysate
Lane 2 : Raji cell lysate
Lane 3 : NCI-H929 cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa
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All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/1000 dilution
Lane 1 : Mouse brain
Lane 2 : Mouse lung
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa
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All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2000 dilution
Lane 1 : Mouse brain
Lane 2 : Rat brain
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa
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All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution
Lane 1 : U87-MG cell lysates
Lane 2 : SK-N-SH cell lysates
Lane 3 : IMR32 cell lysates
Lane 4 : SK-N-AS cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 142 kDa
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Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.
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Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg
Predicted band size: 142 kDa
5% SDS PAGE -
Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.
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Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min