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Anti-DCTN1/p150-glued antibody (ab96004)

Price and availability

291 484 ₸

Availability

Order now and get it on Wednesday February 24, 2021

Anti-DCTN1/p150-glued antibody (ab96004)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)
  • ChIP - Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade (ab1791)

Key features and details

  • Rabbit polyclonal to DCTN1/p150-glued
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-DCTN1/p150-glued antibody
    See all DCTN1/p150-glued primary antibodies
  • Description

    Rabbit polyclonal to DCTN1/p150-glued
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DCTN1/p150-glued aa 1216-1248.
    Database link: NP_004073

  • Positive control

    • Jurkat whole cell lysate (ab7899) for WB. HeLa cells for IF. OV CA for IHC-P.
  • General notes

    Previously labelled as DCTN1. 

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Signal Transduction
    • Cytoskeleton / ECM
    • Cytoskeleton
    • Motor Proteins
    • Dynein
    • Signal Transduction
    • Protein Trafficking
    • Vesicle Transport
    • Regulation
    • Signal Transduction
    • Protein Trafficking
    • ER Proteins
    • Neuroscience
    • Neurology process
    • Growth and Development
    • Axonal Guidance Proteins
    • Signal Transduction
    • Protein Trafficking
    • Golgi Proteins

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)

    Immunohistochemical analysis of paraffin-embedded Rat brain, using ab96004 at 1/500 dilution. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

  • Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution

    Lane 1 : Jurkat cell lysate
    Lane 2 : Raji cell lysate
    Lane 3 : NCI-H929 cell lysate

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/1000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Mouse lung

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2000 dilution

    Lane 1 : Mouse brain
    Lane 2 : Rat brain

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    All lanes : Anti-DCTN1/p150-glued antibody (ab96004) at 1/2500 dilution

    Lane 1 : U87-MG cell lysates
    Lane 2 : SK-N-SH cell lysates
    Lane 3 : IMR32 cell lysates
    Lane 4 : SK-N-AS cell lysates

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG antibody

    Predicted band size: 142 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)

    Immunofluorescence analysis of methanol-fixed HeLa, using ab96004 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled (Red) at 1:2000.

  • Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    Western blot - Anti-DCTN1/p150-glued antibody (ab96004)
    Anti-DCTN1/p150-glued antibody (ab96004) at 1/3000 dilution + Jurkat whole cell lysate at 30 µg

    Predicted band size: 142 kDa



    5% SDS PAGE
  • Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (ab96004)
    Immunocytochemistry/ Immunofluorescence - Anti-DCTN1/p150-glued antibody (ab96004)
    Immunofluorescence analysis of methanol-fixed HeLa cells, using ab96004 at 1/200 dilution. Lower image costained with Hoerchst 33342.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DCTN1/p150-glued antibody (ab96004)

    Immunohistochemical analysis of paraffin-embedded ovarian cancer, using ab96004 at 1/100 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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