Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
![Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)](https://www.abcam.com/ps/products/223/ab223895/Images/ab223895-332329-anti-myc-tag-antibody-9e10-alexa-fluor-647-flow-cytometry.jpg "Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)")
Key features and details
- Alexa Fluor® 647 Mouse monoclonal [9E10] to Myc tag
- Suitable for: ICC/IF, Flow Cyt
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
- Isotype: IgG1
Overview
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Product name
Alexa Fluor® 647 Anti-Myc tag antibody [9E10]
See all Myc tag primary antibodies -
Description
Alexa Fluor® 647 Mouse monoclonal [9E10] to Myc tag -
Host species
Mouse -
Conjugation
Alexa Fluor® 647. Ex: 652nm, Em: 668nm -
Specificity
This antibody is specific for Myc tagged proteins. The Myc tag epitope (EQKLISEEDL) is located at the dimerization site of c-myc and therefore this antibody does not perform well at recognizing endogenous c-myc. A publication by Baker AM et al. 2016 (PMID: 26826706 DOI: 10.1111/his.12939) shows the IHC staining generated by the 9E10 clone does not correlate with c-myc mRNA expression. -
Tested applications
Suitable for: ICC/IF, Flow Cytmore details -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Epitope
aa 410-419 of human Myc. -
Positive control
- ICC/IF: 293T cells transfected with TMEM119 with myc tag Flow Cyt: 293T cells transfected with TMEM119 with myc tag
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
9E10 -
Myeloma
Sp2/0 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Images
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Flow Cytometry - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)
Flow cytometry analysis of HEK-293T (human embryonic kidney) transfected with Myc-tagged TMEM119 expression vector labelling Myc-tag with ab223895 at 1/500 dilution (right) compared with Mouse IgG1 (monoclonal) Alexa Fluor® 647 (left). Cells were surface-stained with TMEM119 antibody (ab210405), then fixed with 2% PFA for 10 minutes and permeabilised with 0.1% Tween-20 for 30 minutes. Next, they were stained with Alexa Fluor® 647 conjugated Myc-tag antibody (ab223895) and Alexa Fluor® 488 conjugated anti-Rabbit secondary antibody (for TMEM119). Only TMEM119 (+) population showed Myc-tag positive staining.
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![Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)](https://www.abcam.com/ps/products/223/ab223895/Images/ab223895-332328-anti-myc-tag-antibody-9e10-alexa-fluor-647-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Alexa Fluor® 647 Anti-Myc tag antibody [9E10] (ab223895)ab223895 staining Myc-tag (pseudocolored in white) in 293T cells transfected with TMEM119 with myc tag. The cells were fixed with 4% formaldehyde (10 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab223895 at 1/100 dilution and ab195889, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 594), at 1/250 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue) and Alexa Fluor® 488 conjugated control Myc-tag antibody (different clone) is shown in green.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
