Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629)
![Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629)](https://www.abcam.com/ps/products/234/ab234629/Images/ab234629-343459-ab231078338947ab231078AP43593515pcMilkPublish.jpg "Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629)")
Key features and details
- Mouse monoclonal [7C12] to C3b / iC3b - BSA and Azide free
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-C3b / iC3b antibody [7C12] - BSA and Azide free
See all C3b / iC3b primary antibodies -
Description
Mouse monoclonal [7C12] to C3b / iC3b - BSA and Azide free -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Full length native protein (purified) corresponding to Human C3b/ iC3b. C3b(i)-Sepharose. C3bi was deposited on Sepharose 4B via the alternative pathway of complement activation in normal human serum.
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Positive control
- WB: human serum and plasma lysates, human liver whole tissue lysate
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General notes
This antibody clone is manufactured by Abcam. If you require a different buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com
ab234629 is a PBS-only version of ab231078.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
7C12 -
Isotype
IgG1 -
Light chain type
kappa
Images
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Western blot - Anti-C3b / iC3b antibody [7C12] - BSA and Azide free (ab234629)All lanes :
Lane 1 : Human serum diluted 1/100 at 5 µl
Lane 2 : Human plasma diluted 1/100 at 5 µl
Lane 3 : Human liver whole tissue lysate at 20 µg
Performed under reducing conditions.
Predicted band size: 187 kDa
Additional bands at: 42 kDa (possible cleavage fragment)This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 55 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% milk before ab231078 and ab181602 (Rabbit anti GAPDH) were incubated overnight at 4°C at a 1ug/ml concentration and 1/10000 dilution respectively. Antibody binding was detected using Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/20000 dilution for 1 hour at room temperature before imaging.
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (ab231078).
