Anti-XIAP antibody (ab137392)
Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-XIAP antibody
See all XIAP primary antibodies -
Description
Rabbit polyclonal to XIAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rabbit, Pig -
Immunogen
Recombinant fragment, corresponding to a region within amino acids 282-470 of Human XIAP (Uniprot ID: P98170).
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Positive control
- A549 and NIH 3T3 whole cell lysates; HeLa cells; Human SW480 xenograft tissue.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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All lanes : Anti-XIAP antibody (ab137392) at 1/500 dilution
Lane 1 : WT HeLa cell extract
Lane 2 : XIAP Knockout 293T cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG
Predicted band size: 57 kDa
Observed band size: 73 kDa why is the actual band size different from the predicted?
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Anti-XIAP antibody (ab137392) at 1/1000 dilution + NIH 3T3 whole cell lysate
at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE -
Immunofluorescence analysis of paraformaldehyde-fixed HeLa cells, labelling XIAP with ab137392 at 1/200 dilution. Lower image is merged with DNA probe.
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Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, labelling XIAP with ab137392 at 1/500 dilution.
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Anti-XIAP antibody (ab137392) at 1/1000 dilution + A549 whole cell lysate at 30 µg
Predicted band size: 57 kDa
7.5% SDS PAGE