Anti-XIAP antibody (ab233164)
Key features and details
- Rabbit polyclonal to XIAP
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-XIAP antibody
See all XIAP primary antibodies -
Description
Rabbit polyclonal to XIAP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat
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Immunogen
Recombinant full length protein (His-tag) corresponding to Human XIAP aa 1 to the C-terminus. Expressed in E.coli.
Database link: P98170 -
Positive control
- WB: Recombinant human XIAP; HepG2 and HEK-293T whole cell lysate; human heart and liver tissue lysate. IHC-P: Human kidney tissue. ICC/IF: HepG2 cells.
Properties
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Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-XIAP antibody (ab233164)Anti-XIAP antibody (ab233164) at 1 µg/ml + Recombinant human XIAP
Secondary
HRP-Linked Guinea pig Anti-Rabbit Ab at 1/2000 dilution
Predicted band size: 57 kDa
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-XIAP antibody (ab233164)Formalin-fixed, paraffin-embedded human kidney tissue stained for XIAP using ab233164 at 20μg/ml in immunohistochemical analysis.
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Immunocytochemistry/ Immunofluorescence - Anti-XIAP antibody (ab233164)HepG2 (human liver hepatocellular carcinoma cell line) cells stained for XIAP (green) using ab233164 at 20μg/ml in ICC/IF. FITC staining.
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Western blot - Anti-XIAP antibody (ab233164)All lanes : Anti-XIAP antibody (ab233164) at 1 µg/ml
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Human heart tissue lysate
Lane 4 : Human liver tissue lysate
Secondary
All lanes : HRP-Linked Guinea pig Anti-Rabbit at 1/2000 dilution
Developed using the ECL technique.
Predicted band size: 57 kDa
