Anti-XIAP antibody [EPR22189-113] (ab229050)
![Anti-XIAP antibody [EPR22189-113] (ab229050)](https://www.abcam.com/ps/products/229/ab229050/Images/ab229050-316592-anti-xiap-antibody-epr22189-113-western-blot.jpg "Anti-XIAP antibody [EPR22189-113] (ab229050)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR22189-113] to XIAP
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Overview
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Product name
Anti-XIAP antibody [EPR22189-113]
See all XIAP primary antibodies -
Description
Rabbit monoclonal [EPR22189-113] to XIAP -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species WB MouseRatHuman
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Immunogen
Recombinant full length protein. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293T, HeLa, WI-38, HepG2, A549, C6, RAW 264.7, PC-12, NIH/3T3 and Neuro-2a whole cell lysates.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR22189-113 -
Isotype
IgG -
Research areas
Images
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Western blot - Anti-XIAP antibody [EPR22189-113] (ab229050)All lanes : Anti-XIAP antibody [EPR22189-113] (ab229050) at 1/1000 dilution
Lane 1 : C6 (rat glial tumor cell line) whole cell lysate
Lane 2 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell lysate
Lane 4 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate
Lane 5 : Neuro-2a (mouse neuroblastoma cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 50-56 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-XIAP antibody [EPR22189-113] (ab229050)](https://www.abcam.com/ps/products/229/ab229050/Images/ab229050-316591-anti-xiap-antibody-epr22189-113-western-blot.jpg)
Western blot - Anti-XIAP antibody [EPR22189-113] (ab229050)All lanes : Anti-XIAP antibody [EPR22189-113] (ab229050) at 1/1000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : WI-38 (human fetal lung fibroblast cell line) whole cell lysate
Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 5 : A549 (human lung carcinoma cell line) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 56 kDa
Observed band size: 50-56 kDa why is the actual band size different from the predicted?
Exposure time: 37 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
The expression profile observed is consistent with what has been described in the literature (PMID: 25535897).
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![Anti-XIAP antibody [EPR22189-113] (ab229050)](https://www.abcam.com/ps/products/229/ab229050/Images/ab229050-3-benefits-of-recombinant-antibodies.png)
Anti-XIAP antibody [EPR22189-113] (ab229050)
