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Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP4863(2)] to Melanoma gp100 - Low endotoxin, Azide free
  • Suitable for: ICC, IHC-P, Flow Cyt, IP, WB
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free
    See all Melanoma gp100 primary antibodies

  • Description

    Rabbit monoclonal [EP4863(2)] to Melanoma gp100 - Low endotoxin, Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: ICC, IHC-P, Flow Cyt, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate and Human melanoma lysate. IHC: Human melanoma tissue. ICC: B16-F0 (Mouse melanoma epithelial cell-like) cell line. Flow cyt: B16-F0 (Mouse melanoma epithelial cell-like) cell line. IP: B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate

  • General notes

    ab193201 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Images

  • Flow Cytometry - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Flow Cytometry - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)

    Overlay histogram showing B16-F0 (Mouse skin melanoma cell) cells stained with ab137078 (red line). The cells were fixed with 4% paraformaldehyde and then permeabilized with 90% methanol. The cells were then incubated with ab137078 at 1/40 dilution. The secondary antibody used wasGoat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution. Isotype control antibody (black line) was Rabbit monoclonal IgG (ab172730). Unlabelled control (blue line) was cells without incubation with primary antibody and secondary antibody.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Immunocytochemistry - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Immunocytochemistry - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)

    ab137078 staining Melanoma gp100 in B16-F0 (mouse skin) cells by ICC (Immunocytochemistry). Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody at a dilution of 1/50. A goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a dilution of 1/1000. Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) ab195889 was used as a counterstain at a dilution of 1/200. DAPI was used as a nuclear counterstain.

     This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)

    ab137078 staining Melanoma gp100 in human melanoma tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and antigen retrieval was by heat mediation in a EDTA buffer. Samples were incubated with primary antibody at a dilution of 1/250 for 30 minutes at room temperature. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody.

    The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument.

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/10000 dilution + B16-F0 (Mouse melanoma epithelial cell-like) whole cell lysate 15µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution


    Exposure time: 50 seconds

    Observed MW: 85-100 KDa

    The molecular weights observed are consistent with what have been described in PMID: 16492709 and PMID: 7961886

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Western blot - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Anti-Melanoma gp100 antibody [EP4863(2)] (ab137078) at 1/1000 dilution + Human melanoma lysate 15µg

    Secondary
    Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution


    Exposure time: 18-0 seconds

    Observed MW: 85-100 KDa

    The molecular weights observed are consistent with what have been described in PMID: 16492709 and PMID: 7961886

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Immunoprecipitation - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Immunoprecipitation - Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)

    ab137078 immunoprecipitating Melanoma gp100. 10µg of cell lysate was incubated with primary antibody at a dilution of 1/500 and VeriBlot for IP Detection Reagent (HRP) (ab131366) at a dilution of 1/10000.

    Lane 1: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate 10ug
    Lane 2: B16-F0 (mouse melanoma epithelial cell-like) whole cell lysate
    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab137078 in B16-F0 whole cell lysate

    Exposure time: 1 second

    Observed MW: 85-100 KDa

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab137078).

  • Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)
    Anti-Melanoma gp100 antibody [EP4863(2)] - Low endotoxin, Azide free (ab193201)

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