Biotin Anti-Melanoma gp100 antibody [EP4863(2)] (ab204674)
![Biotin Anti-Melanoma gp100 antibody [EP4863(2)] (ab204674)](https://www.abcam.com/ps/products/204/ab204674/Images/ab204674-258588-anti-melanoma-gp100-antibody-ep48632-biotin-immunohistochemistry.jpg "Biotin Anti-Melanoma gp100 antibody [EP4863(2)] (ab204674)")
Key features and details
- Biotin Rabbit monoclonal [EP4863(2)] to Melanoma gp100
- Suitable for: IHC-P
- Reacts with: Human
- Conjugation: Biotin
- Isotype: IgG
Overview
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Product name
Biotin Anti-Melanoma gp100 antibody [EP4863(2)]
See all Melanoma gp100 primary antibodies -
Description
Biotin Rabbit monoclonal [EP4863(2)] to Melanoma gp100 -
Host species
Rabbit -
Conjugation
Biotin -
Tested Applications & Species
See all applications and species dataApplication Species IHC-P Human
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Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human skin melanoma tissue
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Store In the Dark. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 30% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Affinity purified -
Clonality
Monoclonal -
Clone number
EP4863(2) -
Isotype
IgG -
Research areas
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Biotin Anti-Melanoma gp100 antibody [EP4863(2)] (ab204674)
IHC image of Melanoma gp100 staining in a section of formalin-fixed paraffin-embedded human skin melanoma*. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins, performed on a Leica BONDTM. The section was then incubated overnight at +4°C with ab204674 at 1/50 dilution. Detection was via an HRP conjugated ABC system and DAB was used as the chromogen (ab103723), diluted 1/100 and incubated for 10min at room temperature. The section was then counterstained with haematoxylin and mounted with DPX.
The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
